HLA—A、B基因多态性与遵义地区汉族人群肾综合征出血热的相关性研究

Correlation between the HLA-A, B alleles polymorphism and hemorrhagic fever with renal syndrome of Han nationality in Zunyi area

目的探讨HIA—A、B基因多态性与遵义地区汉族人群肾综合征出血热（HFRS）的关联性。方法采用群体研究方法，应用聚合酶链反应-序列特异性引物（PCR—SSP）技术对100例肾综合征出血热患者和100例健康对照者进行HLA—A、B基因分型，比较其等位基因频率（GF），并计算其相对危险度（RR）。结果肾综合征出血热患者组中，HLA—A＊31、B＊58的等位基因的基因频率分别为4％、12．5％，较健康对照组的0.5％明显增高，两组比较差异具有统计学意义（X^2值分别为6．380和7．792，P〈0．05，RR值分别为18．47、2．91）；患者组中HLA—B＊40等位基因的基因频率（11％）显著低于健康对照组（19％），两者之间差异具有统计学意义（X^2=6．095，P〈0．01，RR值为0．47）。结论研究提示在遵义地区汉族人群中，初步认为HLA—A＊31、B＊58基因与HFRS呈正相关，HIA—B＊40基因与HFRS呈负相关。

Objective To explore the correlation between HLA-A, B alleles polymorphism and hemorrhagic fever with renal syndrome （HFRS） among Han nationality in Zunyi area. Methods Using group study, HLA-A, B genotypes were conducted in 100 HFRS cases and 100 controls among Han nationality in Zunyi area with polymerase chain reaction-sequence specific primer（ PCR-SSP）, gene frequency（GF） and relative risk（RR） were compared and calculated. Results The frequencies of HLA-A ＊ 31 and HLA-B＊58 alleles in HFRS cases （ GF = 4% , 12.5% ） were strikingly higher than that in the healthy controls （ X^2 = 6. 380, 7. 792, P 〈 0.05 ; RR = 18.47, 2.91 ）. The frequencies of HLA-B ＊ 40 alleles in HFRS cases （GF = 11%） were strikingly higher than that in the healthy controls （ X^2 =6.095, P 〈0. 01, RR = 0.47）. Conclusion HLA-A ＊ 31, B ＊ 58 genes are positively related to HFRS of Han nationality in Zunyi area, HLA-B ＊ 40 gene is negatively related to HFRS of Han nationality in Zunyi area.