急性脑梗塞患者血小板膜糖蛋白的研究

Platelet membrane glycoproteins in acute cerebral infarction patients

目的 本研究分析急性脑梗塞患者及对照组血小板膜糖蛋白(GP)Ⅱb-Ⅲa、GPⅠb、α-颗粒膜蛋白(GMP-140)和假血管性血友病因子(vWF)的改变,并讨论了GPs在脑缺血发病中的重要意义。方法 应用活化依赖的单克隆抗体和流式细胞术(FCM)。结果 病例组阳性细胞百分率和平均荧光强度均较对照组明显升高(P<0.001～0.05)。结论 急性脑梗塞患者血小板处于明显激活状态。GPs为血小板活化和血栓形成的特异性分子标志物,为药物干预的靶子。FCM为检测脑缺血患者血小板活化程度和抗血小板治疗疗效提供了一种新方法及一条新颖的抑制血小板聚集的途径。

The level of glycoprotein(GP) II b- IIIa, GPIb, 140 kd α-granule membrane proteinCGMP-140) and von willebrand factor (vWF) on platelets from pateints with acute cerebral infarction were measured and compared with the platelets obtained from control subjects. Methods Activation-dependent monoclonal antibodies and flow cytometry were used. Results The percentage of positive cells and the mean fluorescence intensity of activated platelets in stroke patients were significantly increased as compared with that in control subjects( P <0. 001-0. 05). Conclusion These results suggested that platelets of patients with a-cute cerebral infarction became markedly activated. GPs are specific molecular markers for platelets activation and thrombosis,representing targets for pharmacological intervention and a novel approach to the inhibition platelet aggregation. Flow cytometry provided a new method for investigating the degree of platelet activation and the efficacy of antiplatelet therapy in ischemic stroke patients.