荧光分子层析中的全时间分辨图像重建法

Image Reconstruction in Fluorescence Molecular Tomography Based on Full Time-Resolved Scheme

在荧光分子层析(Fluorescence molecular tomography,FMT)中,全时间分辨(Time Resolved,TR)测量包含了最多的光子传输信息。基于有限元-有限差分扩散方程的正向模型和Newtown-Raphson的逆向模型,将全时间分辨方法用于时域荧光分子层析中,用模拟数据对算法在空间分辨率、定量性、重建尺寸和灰度的保真度以及噪声稳健性等方面进行了验证。结果表明,此方法能够实时重建荧光产率和荧光寿命图像。与以前发展的基于广义脉冲谱技术(Generalized pulse spectrum technique,GPST)的特征数据法进行图像重建相比较,整体上优于广义脉冲谱技术。

In fluorescence molecular tomography （FMT）, the time-resolved （TR） measurement can provide the richest information on photon migration. A full time-resolved scheme is extended to time-domain fluorescence molecular tomography, based on the finite element-finite time difference diffusion equation in forward model and the Newtown-Raphson inversion method. The abilities of spatial resolution, quantitativenesss, discerning the differences in target size, grayscale resolution and noise robustness are validated by using simulated data. The proposed method is demonstrated with the capability of simultaneously recovering the fluorescent yield and lifetime. Compared with the generalized pulse spectrum technique for reconstructing images, it shows its superiority in almost every aspect.