豆油对饲克山病病区粮大鼠抗氧化能力的影响

INFLUENCE OF SOYBEANOIL ON ANTIOXIDATIVE ABILITY OF RATS FED ON CEREALS FROM KESHAN DISEASE AREA

以非病区粮对照、克山病病区粮及其添加豆油（６０ｇ豆油／ｋｇ饲料）饲料喂养大鼠７０ｄ，测定各组织中维生素Ｅ及血浆中脂质过氧化物含量。结果显示：无论是病区粮饲料组，还是非病区粮饲料组，在饲料中添加豆油后，动物血浆中α－生育酚（α－Ｔｏｃｏｐｈｅｒｏｌ）含量均增加，而心肌线粒体中α－Ｔｏｃｏｐｈｅｒｏｌ含量则下降，肝线粒体中α－Ｔｏｃｏｐｈｅｒｏｌ和γ－Ｔｏｃｏｐｈｅｒｏｌ含量均下降，红细胞膜中α－Ｔｏｃｏｐｈｅｒｏｌ含量亦有降低趋势。另外，饲料中长期添加豆油，可使大鼠血浆中脂质过氧化物含量显著增加。说明，随着多不饱和脂肪酸（ＰＵＦＡｓ）摄入量的增加，膜系统α－Ｔｏｃｏｐｈｅｒｏｌ的消耗量亦增加，ＰＵＦＡｓ长期负荷，可使饲克山病病区粮（低硒低维生素Ｅ）饲料动物体内的抗氧化能力下降。

Rats were kept on Keshan disease(KD)endemic or nonendemic grains for 70 days.The results of VE contents in tissues and LPO contents in plasma showed:When soybean oil was added to either endemic or nonendemic cereals(60g soybean oil/kg cereals),αTocopherol contents of plasma were increased,but αTocopherol in myocardial mitochondria,α,γTocopherol in liver mitochondria and αTocopherol in red cell membrane were all decreased.LPO contents of plasma in rats fed on endemic cereals were significantly higher than those fed on nonendemic cereals.These results demonstrated that the amount of αTocopherol used up in membrane increased with the increased amount of polyunsaturated fatty acids intake from cereals,and polyunsaturated fatty acids longterm overload could decrease the antioxidative ability of rats fed on cereals from KD endemic area.