高效液相色谱法测定散寒解热颗粒剂中葛根素的含量

HPLC Determination of Puerarin in Sanhanjiere Granules

目的：建立散寒解热颗粒剂中葛根素的含量测定方法。方法：样品用甲醇超声提取,采用高效液相色谱法,Nuc leosil-C18（200mm×4.6mm,7μm）分析柱,乙腈∶水（内含0.02moL/L磷酸二氢钠用10%磷酸调pH值为4.0）（12.5∶87.5）为流动相,检测波长265nm。结果：葛根素峰的保留时间约为13min。并与其邻近峰的分离度均大于1.5,葛根素的线性范围为0.4-1.4μg（r=0.9999）,平均回收率为98.7%（n=5）。结论：方法简便,快速,结果准确可靠,可作为散寒解热颗粒剂中葛根素的含量测定方法。

Objective：To establish a method for determining the concentration of puerarin in Sanhanjiere granules.Methods：The sample was extracted with methanol on a supersonic.HPLC was used for the determination of puerarin in Sanhanjiere granules.The chromatographic procedure was carried out using Nucleosil-C18（200mm×4.6mm,7μm） as an analytical column and a mixture of 12.5 volume of acetonitrile,87.5 volume of water which was contained 0.02moL/L Sodium dihydrogen phosphate and adjusted the solution pH4.0 by phosphoric acid as mobile phase.The detection wavelength was set at 265nm.Results： The peaks of puerarin appeared on about 13 minutes and the resolutions of them were more than 1.5.Their linear ranges were between 0.4 and 1.4g（r=0.9999）,with the average recoveries of 98.7%（n=5）.Conclusion：This method is sensitive,quick and accurate for the measurement of puerarin in Sanhanjiere granules.