从制革废灰碱牛皮中提取天然胶原(Ⅱ)——胶原的物理化学性能表征

Extracting of Native Collagen from Bovine Limed Split Wastes:Physicochemical Properties of Obtained Collagen

对经EDTA和盐酸预处理后提取得到的天然胶原的物理化学性能进行了表征。经盐酸预处理后提取得到的胶原(HPC)的等电点(5.38±0.02),低于经EDTA预处理后提取得到的胶原(EPC)的等电点(5.87±0.03),HPC的自由氨基含量低于EPC;同时,HPC的平均粒径和相对黏度均大于EPC。两者的氨基酸组成相似,与商品小牛皮胶原(CCC)差别不大;通过电泳、圆二色谱分析分别验证了EPC和HPC的多肽链和三股螺旋结构的完整性。差式扫描量热法分析表明:EPC和HPC与商品小牛皮胶原一样,具有较好的热稳定性。

The physicochemical properties of collagen extracted from EDTA - and HCl - pretreated bovine limed split wastes respectively were charactered. The isoelectric point of HPC （ 5.38 ± 0.02） is lower than that of EPC （5.87 ± 0.03 ）. And the primary amino acid content of HPC is lower than that of EPC. Simultaneity, HPC has larger Z - particle size and higher relatively viscosity than those of EPC. The amino acid compositions of EPC and HPC are similar to each other, although there is a little difference compared to that of commercial calfskin collagen （CCC）. The results of sodium dodecyl sulphate polyacrylamide gel electrophoresis analysis （SDS -PAGE） and circular dichroism （CD） spectras represented the integralities of their polypeptide and triple helix conformations. Thermal behaviors of EPC and HPC, reviewed using differential scanning calorimetry （ DSC）, are almost as same as that of CCC.