自杀基因系统联合六味地黄丸对肝癌细胞杀伤的协同作用

Synergistic Killing Action of Suicide Gene System Combined with Medicated Serum of Liuwei Dihuang Bolus on Hepatoma Cells

【目的】观察六味地黄丸含药血清协同HSV-tk/GCV自杀基因治疗系统杀伤大鼠肝癌CBRH7919细胞的作用,探讨建立自杀基因联合中药方药的肿瘤基因治疗联合方案的可行性。【方法】构建HSV-tk/GCV自杀基因治疗系统,确定丙氧鸟苷(GCV)工作浓度(39.2μmol/L);选用SD大鼠灌胃制备六味地黄丸含药血清和空白血清,并观察含药血清和空白血清对肿瘤细胞生长的影响。设空白对照组、GCV对照组、自杀基因治疗对照组,以上3组均为无大鼠血清的对照组。空白血清组、GCV加空白血清组、自杀基因系统加空白血清组、含药血清组、GCV加含药血清组、自杀基因系统联合含药血清组中,所有大鼠的空白血清和含药血清再分为体积分数5%和7.5%2种浓度,每组设6个复孔。将大鼠肝癌细胞CBRH7919/tk+和CBRH7919/tk-混合,使tk+细胞的比例分别占0%、5%、10%,按3×103个/孔细胞分别接种到96孔板,用完全培养基培养细胞24h后,相应加入体积分数5%或7.5%的大鼠空白血清和含药血清,孵育12h,加入GCV,培养60h,四甲基偶氮唑盐(MTT)法检测细胞存活率,用Q值(实测药效与理论药效的比值)分析自杀基因系统与含药血清联合的相互作用是否具有协同性(0.85≤Q<1.15为相加作用,Q≥1.15为协同作用)。【结果】空白血清和含药血清浓度在体积分数20%以下时未显示明显细胞毒性;血清浓度为体积分数5%和7.5%时,自杀基因系统联合含药血清组与单独含药血清组及自杀基因系统加空白血清组比较,联合组对肿瘤细胞的杀伤作用均强于其他非联合组,细胞存活率均有显著性差异(P<0.01),而且自杀基因系统联合含药血清的作用具有明显的协同性(Q>1.15)。【结论】HSV-tk/GCV自杀基因治疗系统联合六味地黄丸对杀伤肿瘤细胞具有协同增效作用。

Objective To investigate the possibility of establish a combined regimen of Chinese medicine and suicide gene therapy, we observed the killing action of medicated serum of Liuwei Dihuang Bolus （LDB） combined with HSV-tk/GCV suicide gene therapy system on rats hepatoma cell line CBRH7919. Methods The HSV-tk/GCV suicide gene therapy system was constructed and the working solution of ganciclovir （GCV） at 39.2 μmol/L was detected firstly. Then medicated or non-medicated serum was prepared from SD rats treated with or without LDB by gavage. Meanwhile, the effects of medicated and non-medicated serum on cell proliferation were detected. The control groups without serum added were blank control group, GCV group and suicide gene therapy （SGT） group. Blank serum groups, blank serum ＋ GCV groups, blank serum ＋ SGT groups, medicated serum groups, medicated serum ＋ GCV groups and medicated serum ＋ SGT groups all had two serum concentrations of 5% and 7.5% , respectively. Six double holes were set for each group. CBRH7919/tk^＋ and CBRH7919/tk^- were mixed together, and the tk^＋ cell percentage was adjusted to 0% , 5% and 10% , respectively. Then the mixture of CBRH7919/tk^＋ and CBRH7919/tk^- was implanted into the 96-hole plates at a density of 3 × 10^3 cells/ hole, cultured with complete medium for 24 hours, and then treated with medicated or non-medicated serum for 12 hours and sequentially with GCV at 39. 2 μmol/L for another 60 hours. The number of surrival cells was determined by MTT assay. Q value （the ratio of measured and theoretical pharmacological action） was used to analyze the synergism of Chinese medicine and suicide gene therapy： additive action when 0. 85 ≤ Q 〈 1.15 and synergistic action when Q 〉 1.15. Results No cytotoxicity was found when blank serum or medicated serum was below the concentration of 20% （volume fraction）. When the concentration of serum was at 5% and 7.5% , the killing action of SGT ＋ medicated serum group was stronger and the cell survival rate was higher those that of medicated serum group and SGT ＋ blank serum group （P 〈 0. 01 ）. And SGT combined medicated serum showed an obvious synergistic action （Q 〉 1.15）. Conclusion HSV-tk/GCV suicide gene therapy system combined with LDB has a synergistic killing action on rats hepatoma cells.