慈竹叶片黄酮的提取工艺及在四川不同地区含量的差异

Techniques flavone extraction from leaves and differences of flavone content in Neosinocalamus affinins from different regions of Sichuan Province

【目的】优化慈竹叶片黄酮的乙醇提取工艺,揭示四川不同地区慈竹及其不同部位黄酮含量的差异,为高含量黄酮慈竹的栽培提供理论依据。【方法】设置乙醇体积分数、料液比、提取温度和提取时间4个因素,采用L9(34)正交试验,对西南科技大学校园内慈竹叶片黄酮的提取工艺进行了优化,采用NaNO2-Al(NO3)3比色法测定黄酮含量。最后采用优化后的慈竹黄酮提取工艺,对四川省5个不同地区的慈竹完全展开叶片、幼笋和大笋黄酮含量进行了测定和比较分析。【结果】(1)乙醇体积分数对慈竹叶片黄酮提取的影响程度最大,其次是提取时间,料液比对黄酮的提取的影响程度最小。(2)慈竹叶片黄酮含量高于幼笋和大笋。(3)四川不同地区慈竹各部位的黄酮含量存在差异,叶片黄酮含量以眉山市青神县的慈竹最高,雅安市芦山县最低;幼笋和大笋的黄酮含量以达州市大竹县和宜宾市长宁县的最高。【结论】乙醇提取慈竹叶片黄酮的适宜工艺为体积分数70%乙醇溶液,料液比为1∶20,在70℃条件下提取2 h。眉山市青神县栽培的慈竹是提取叶片黄酮的良好原料。

[Objective] The suitable technology of ethanol extraction in the flavone from leaves in Neosinocalamus affinins was studied. The differences of flavone content in Neosinocalamus affinins from different regions of Sichuan were revealed,and the theoretical evidence was provided for culturing Neosinocalamus affinins with the high flavone content. [Method] The suitable techniques of flavone extraction were studied through four factors,including ethanol concentration,ratio of sample with ethanol solution, extraction temperature and time, using the mature leaves of Neosinocalamus affinins in Southwest University of Science and Technology. The design of orthogonal experiment L9 （34 ） was used to select the suitable techniques of extracting flavone from the leaves of Neosinocalamus affinins. The flavone content was determined by NaNO2-Al（NO3）3 colorimetric method. The flavone of the leaves, young shoots, old shoots in Neosinocalamus affinins in different regions of Sichuan was extracted through the suitable techniques. [Result] （1）The effect of ethanol concentration on the flavone extraction of leaves from Neosinocalamus a f finins was significant and higher than that of the others. The second was the time extracting flavone of leaves. There was no significant ratio of samples with ethanol solution on the flavone extraction. （2）The flavone content of mature leaves in Neosinocalamus affinins was higher than that of young and old shoots through the comparative analysis of flavone contents from the mature leaves and young shoot and old shoots. （3）The differences of flavone content among them were found in different regions. The flavone contents of mature leaves from MSQ and YAL were the highest and the lowest respectively. The flavone contents of young shoots from DZD and old shoots from YBC were the highest. [Conclusion] The techniques of flavone extraction were suitable under the ratio of 20 folds of 70% of ethanol with 1 fold of sample at 70℃ for 2 hours. The leaves of Neosinocalamus affinins in MSQ were suitable for extracting the flavone.