猫贲门失弛缓症模型肌间神经丛的超微结构研究

Ultrastructure study on myenteric nerve plexus of achalasia models

目的应用苯基二甲基十四烷氯化铵(BAC)建立猫贲门失弛缓症模型,研究其下食管括约肌(LES)肌间神经丛的微观结构。方法10只猫随机分为两组,模型组胃镜下LES处环形注射BAC,对照组注射生理盐水,8周后检测食管动力学变化,然后应用电子显微镜观察LES肌间神经丛的形态学变化。结果模型组LES压力较对照组明显升高(P<0.01),两组食管收缩幅度无显著性差异;神经终末内有多种不同类型的突触小泡,两组神经末梢内清亮小泡的数量无显著差异(P>0.05),模型组突触小泡的数量较对照组明显减少(P<0.05)。结论肌间神经丛的突触小泡的减少可能是贲门失弛缓症的发病原因之一。

Objective To explore the microstructure of myenteric nerve plexus in lower esophageal sphincter （LES）, Benzyldimethyhetradecylammonium chloride （BAC） were used to create amyenteric cat achalasia models, Methods Ten cats were randomized into two groups： injected with BAC in models group, saline in controlled group in the distal esophagus with endoscopy respectively, 8 weeks later, manometry were performed. The morphology of the neurons with the myenteric plexus was studied by electron microscope. Results Manometrically, the LES of models cats showed higher pressures than that of the controlled animals （P〈0.01）, The esophageal contraction amplitudes were similar in both groups; there were different types of nerve terminal endings with various of synatic vesicles, The frequency percentage of small agranular vesicles had no differences between two groups （P〉0.05）, The large granular vesicles in models were fewer than that in controls （P〈0.05）. Conclusion The decrease of large granular vesicles in myenteric nerve plexus is one of the important causes of achalasia.