Heparanase-1在恶性嗜铬细胞瘤中的表达及其意义

Expression of Heparanase-1 in malignant pheochromocytomas

目的:探讨类肝素酶(Heparanase)成为一种预判肾上腺恶性嗜铬细胞瘤指标的可能性。方法:选取经手术治疗且具有完整的临床、病理和随访资料的肾上腺嗜铬细胞瘤患者的存档石蜡标本27例,其中良性嗜铬细胞瘤10例(良性组),恶性嗜铬细胞瘤17例(恶性组)。另取5例因良性肾疾患行肾切除时获取的同侧正常肾上腺组织作为对照组。采用免疫组织化学技术检测良、恶性嗜铬细胞瘤及正常肾上腺髓质组织中Heparanase-1的表达情况。结果:Heparanase-1在肾上腺恶性嗜铬细胞瘤中表达最高(76.5%),在肾上腺良性嗜铬细胞瘤中表达较低(30.0%),在正常肾上腺髓质组织中无表达,恶性组与良性组及恶性组与正常组之间Heparanase-1的表达差异有统计学意义(P<0.05)。结论:Heparanase-1有望成为预判肾上腺恶性嗜铬细胞瘤的一种指标。

Objective：To analyse the value of Heparanase-1 （HPA） as marker for malignant pheochromocyto-mas. Methods： 27 paraffin-embedded specimens included 10 benign pheochromocytomas and 17 malignant pheo-chromocytomas obtained from operations performed in Rui Jin Hospital affiliated to Shanghai Jiao Tong University School of Medicine from 1986 to 2006 with complete clinical, pathological and follow-up records were selected. Ipsilateral normal adrenal glands from 5 cases underwent nephrectomy for benign renal tumors served as a control group. Immunohistochemical technology was performed to detect the HPA in all specimens. Results：In malignant pheochromocytomas, （76.5%） the expression of HPA was strong, in benign pheochromocytomas the expression was relatively weak （30.0%）, but in the normal adrenal medulla tissue was negative expression. The expression of HPA between the groups of benign pheochromocytoma and malignant pheochromocytoma, malignant pheochro-mocytoma and normall adrenal medulla tissue showed significant differences （P〈0.05）. Conclusions： HPA would become a new predictor marker for malignant pheochromoeytoma.