保健食品中辅酶Q_(10)含量的测定及其提取条件的研究

In health foods coenzyme Q_(10) content determination and extraction condition research

目的：本文建立了测定保健食品中辅酶Q10的高效液相色谱法，并对不同溶剂对辅酶Q10提取效果的影响进行了研究。方法：样品在40℃下，乙酸乙脂超声提取15min，采用Symmetry C18 4．6×250min柱分离；流动相为甲醇＋乙醇（30＋70），样品盘和色谱柱温度均为35℃，流速为1．5ml／min，检测波长275nm。结果：在10-600μg/ml范围内，峰面积与质量浓度成良好的线性关系（r^2=0．999），RSD为0．6％，最低检出限0．8ng。样品中辅酶Q10的提取率〉96％，RSD为2．83％。结论：该方法快速、准确，提取效率高，适用于保健食品中辅酶Q10含量的测定。

Objective：To set up a method of HPLC to detemine the concent of coenzyme Q 10 in health foods and research different resolver to the coenzyme Q10 extraction effect influence. Methods：The sample was extract in 40℃, supersonic 15 min with the ethanoic acid. The separation was performed on a Symmetry C18 4. 6 × 250 mm with the mobile phase consisting of the methyl alcohol ＋ ethyl alcohol （30 ＋ 70）, The sample tray and the chromatographic column temperature is 35℃, The speed of flow is 1.5 ml/min. The decetive wavelength was set at 275 nm. Results：There was a good linear relationship within the range of 10 - 600 μg/ml（r^2 =0. 9999） ,RSD =0. 6%. The detection limit was 0. 8 ng. The extraction rate of sample coenzyme Q10 〉96%. Conclusion：The method is fast, accurate and high extraction efficiency and can be used as a quantitative analysiing method for coenzyme Q10 in health foods.