高效液相色谱法测定水果中的赤霉素、苯甲酸、糖精钠和胭脂红

Determination of gibberellin,benzioc acid,saccharin sodium and ponceau 4R in fruits by high performance liquid chromatography

目的：建立高效液相色谱法测定水果中赤霉素、苯甲酸、糖精钠和胭脂红含量的方法。方法：采用溶剂萃取对样品进行提取，以CH3OH／H2O（NH4Ac-HAc）为流动相，优化流动相配比、流速，采用二极管阵列检测器进行检测（λ=210nm），利用反相高效液相色谱分析法测定水果中赤霉素、苯甲酸、糖精钠和胭脂红。结果：该法线性良好，相关系数r〉0．999，相对标准偏差〈5．0％，加标回收率80％～95％。结论：本方法简单可靠、快速，灵敏度高，定量准确，可用于同时测定水果中赤霉素、苯甲酸、糖精钠和胭脂红。

Objective：A method for the determination of gibberellin, benzoic acid,saccharin sodium and ponceau 4R in the fruits by HPLC was established in this research. Methods ： Samples were extracted by solvent extraction, and mobile phase was CH3OH/H2O （ NH4 Ac - HAc ） = 35 ： 65. Flow rate is 0. 6 ml/min and samples were determined by DAD detector. The column temperature was controlled at 30℃. Results：The method was able to separate and determinate 4 materials in fruits easily and effectively. In the experiment, good relative（ r 〉 0. 999 ）, broad linearity range, low detection limit, good analytic accuracy and precision were obtained （RSD 〈 5% ）. The average recoveries were between 80% and 95%. Conclusion：This method is simple, rapid, sensitive and accurate. It is suitable for simultaneous determination gibberellin, benzioe acid, saccharin sodium and poneeau 4R in fruits.