鸡传染性支气管炎病毒N基因的克隆及序列分析

Clone and sequence analysis of N Gene of an Avian Infectious Bronchitis Virus

根据GenBank上已公布的鸡传染性支气管炎病毒(IBV)N基因序列经多重比较设计一对特异性引物扩增大庆分离株DQ1株的N基因,RT-PCR产物经纯化回收后,克隆到pMD18-T载体上,转化大肠杆菌TG1感受态细胞,筛选出阳性质粒进行序列测定,经与已发表的IBV N基因序列进行序列比较与分析,表明DQ1株的N基因与现有疫苗株存在一定差异。

One pair of primers to amplify the N gene of infectious bronchitis virus （IBV） which was isolated from Daqing was designed and synthesized according to the published results of IBV sequences in Genbank. The production of the RT-PCR was purified and cloned into PMD-18 T vector, then converted to competent cell of the E.coli TG1. Positive plasmids were selected and which sequence was analyzed. The consequence was compared to the published sequences of IBV-N gene in Genbank, the results indicated that the N gene of DQ1 strain was different with the now vaccine strains.