摘要
以猪心脏为原料,确定总磷脂提取的优化条件。设计了以氯仿-甲醇混合溶剂为提取剂的提取工艺试验,探讨了氯仿-甲醇的比例、液料比、水洗比例、提取时间对总磷脂含磷量的影响。并采用二次回归正交旋转组合设计方法,对总磷脂的提取条件进行优化。结果表明,最佳提取条件为:氯仿-甲醇的比例2∶1、液料比22∶1、水洗比例27%、提取时间5h,总磷脂含磷量为20.45mg·mL-1。
Making pig hearts as the material to dtermine the optimum technological condition on extraction of total phospholipids. Chlo- roform-methanol was adopted for extraction, the effect of chloroform to methanol ratio, liquid to material ratio, extraction time and vol- ume percentage of water in washing were investigated. The technological condition was optimized by quadratic regress orthogonal rotary combination design. The results indicated that the optimization condition was as followed： chloroform to methanol ratio was 2：1, liquid to material ratio was 22：1, volume percentage of water in washing was 27 %, extraction time was 5 hours and the phosphorus content was 20.45 mg·mL-1.
出处
《黑龙江八一农垦大学学报》
2008年第3期68-71,共4页
journal of heilongjiang bayi agricultural university
关键词
磷脂
提取
含磷量
phospholipids
extraction
phosphorus content
