HPLC法分别测定芍连胃乐片中芍药苷与盐酸小檗碱含量

Determinination the Contents of Paeoniflorin and Berberine Hydrochloride in Shaolian Weile Tablets by HPLC

目的:建立测定芍连胃乐片中芍药苷和盐酸小檗碱含量的高效液相色谱法。方法:芍药苷含量测定采用C_(18)(200 mm×4.6 mm,5μm),流动相为乙腈:0.05 mol·L^(-1)磷酸二氢钾溶液(15:85),检测波长为230 nm。盐酸小檗碱含量测定采用C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈:0.033 mol·L^(-1)磷酸二氢钾溶液(32:68),检测波长为265 nm。结果:芍药苷在3.0～48.3μg·ml^(-1)范围内线性关系良好(r=1.000 0),平均回收率为98.1%,RSD为0.5%;盐酸小檗碱在1.0～16.4μg·ml^(-1)范围内线性关系良好(r=1.000 0),平均回收率为98.9%,RSD为1.0%。结论:本方法简便、可靠、准确。

Objective： To establish a method for determining the content of paeoniflorin and berberine hydrochloride in Shaolian- Weile tablets by HPLC, respectively. Method： The content of paeoniflorin in Shaolian Weile tablets was determined by HPLC with a C18 column （200 mm×4.6 mm,5μm） , acetonitrile-0.05 mol·L^-1 potassium phosphoric monobasic（ 15： 85） as the mobile phase, detection wavelength was at 230 nm; The content of berberine hydrochloride in Shaolian Weile tablets was determined by HPLC with a C18 column （250 mm ×4.6 mm,5 μm）, acetonitrile-0. 033 mol·L^-1 potassium phosphoric monobasic（32： 68） as the mobile phase, detection wavelength was at 265 nm. Result： The standard curve were linear within the range of 3.0 -48.3 μg of paeoniflorin（ r = 1. 000 0） and 1.0 - 16.4 μg of berberine hydrochloride（r = 0. 999 99） . The average recovery and RSD of paeoniflorin were 98.1% and 0.5%, those of berberine hydrochloride were 98.9% and 1.0%, respectively. Conclusion： The method was simple, accurate, and reproducible.