脉冲Nd:YAG激光照射对实验性大鼠慢性牙周炎的作用

Effect of pulsed Nd :YAG laser irradiation on periodontal pocket in therapy of chronic periodontitis

目的：探讨脉冲Nd：YAG激光照射对大鼠实验性牙周炎牙周临床指标及牙周组织中一氧化氮（NO）含量的影响。方法：健康Sprague—Dawley大鼠共64只，随机分为8组，正常对照组（N组），药物对照治疗组（D组），牙周炎阳性对照组（P组），其余5组按照给予不同剂量激光照射治疗。末次治疗15d后测量牙周临床指标牙龈指数（GI），牙槽骨吸收值ABL（Alver bone loss），菌斑指数（plaque index，PLI），采用分光光度仪测定大鼠牙周组织中NO的含量。结果：各剂量组ABL、NO值较未照射组（P组）显著降低（P〈0．05）。1．5～2．0W 20pps剂量组激光照射后牙周指数均较P组显著降低（P〈0.05），与D组无显著差异。结论：在1．5～2．0W 20pps剂量范围内，采用脉冲Nd：YAG激光牙周袋内照射能有效改善牙周指数，降低实验大鼠牙周组织中NO含量，耗到治疗牙周书的作用。

AIM： To observe the effect of different energy levels of pulsed Nd ：YAG laser irradiation on changes of NO content in periodontal tissue of periodontitis model and changes of periodontal index. METHODS： 64 Sprague -Dawley rates were randomly divided into 8group. 1 group was assigned as the control（N）. 1 group was as- signed as the drug treatment（D）. 1 group was assigned as the positive control of periodontitis（P） . The rest 5 groups were established as the animal model of periodontitis with different energy levels of pulsed Nd ：YAG laser irradiation. Clinical indexes of periodonfitis were detected 15 days after the last treatment. The changes of NO level in the periodontal tissue were assayed by spectrophotometry. RESULTS： Level of ABL, NO in each energy level group was significantly lower than the P group （ P 〈 0.05 ） . The periodontal index with 1.5 - 2.0W 20pps pules Nd ~ YAG laser irradiation group were significantly lower than the P group （ P 〈 0.05）, no significantly change in the drug group. CONCLU- SION ： 1.5W - 2.0W 20pps of pulsed Nd ：YAG laser irradiation on periodontal pocket of the rats can decrease the NO content effectively in periodontal tissue and also can improve the periodontal index.