Composition of inflammatory infiltrate and its correlation with HBV/HCV antigen expression

AIM： To study the composition of liver inflammatory infiltrate in biopsy material from patients chronically infected with hepatotropic viruses and to evaluate the correlation of inflammatory infiltrate with hepatitis B virus （HBV） and hepatitis C virus （HCV） viral antigen expression in chronic B and C hepatitis. METHODS： The phenotype of inflammatory cells was evaluated by the EnVision system, using a panel of monoclonal antibodies. HBV and HCV antigens were detected with the use of monoclonal anti-HBs, polyclonal anti-HBc and anti-HCV antibodies, respectively. RESULTS： The cellular composition of liver inflammatory infiltrate was similar in the patients with B and C hepatitis： ～50%-60% of cells were T helper lymphocytes. Approximately 25% were T cytotoxic lymphocytes; B lymphocytes comprised 15% of inflammatory infiltrate; other cells, including NK, totalled 10%. Expression of HLA antigens paralleled inflammatory activity. Portal lymphadenoplasia was found more often in hepatitis C （54.5%） than in hepatitis B （30.6%）. Expression of HB-cAg was found more often in chronic B hepatitis of moderate or severe activity. Overall inflammatory activity in HBV-infected cases did not correlate with the intensity of HBsAg expression in hepatocytes. Inflammatory infiltrates accompanied the focal expression of HCV antigens. A direct correlation between antigen expression and inflammatory reaction in situ was noted more often in hepatitis C than B. CONCLUSION： Irrespective of the etiology and activity of hepatitis, components of the inflammatory infiltrate in liver were similar. Overall inflammatory activity did not correlate with the expression of HBsAg and HCVAg; HBcAg expression, however, accompanied chronic hepatitis 8 of moderate and severe activity.

瞄准：学习肝的作文煽动性在从长期地感染亲肝病毒并且到的病人的活体检视材料渗入评估关联煽动性与肝炎 B (HBV ) 和丙肝病毒(HCV ) 渗入在长期的 B 和 C 肝炎的病毒的抗原表示。方法：煽动性的房间的显型被 EnVision 系统评估，用单音的同种细胞的抗体的一块面板。HBV 和 HCV 抗原分别地与单音的同种细胞的 anti-HBs， polyclonal anti-HBc 和 anti-HCV 抗体的使用被检测。结果：肝的细胞的作文煽动性渗入在有 B 和 C 肝炎的病人是类似的：房间的 ~50%-60% 是 T 助手淋巴细胞。约 25% 是 T 细胞毒素的淋巴细胞；B 淋巴细胞包括了中的 15% 个煽动性渗入；包括 NK，另外的房间总计了 10% 。HLA 抗原 paralleled 的表示煽动性的活动。门 lymphadenoplasia 比在肝炎 B (30.6%) 在丙肝(54.5%) 更经常被发现。HBcAg 的表示在中等或严重的活动的长期的 B 肝炎更经常被发现。在感染 HBV 的情况中的全面煽动性的活动没在 hepatocytes 与 HBsAg 表示的紧张相关。煽动性渗透伴随了 HCV 抗原的焦点的表示。在抗原表示和炎性反应原位之间的直接关联比 B 在丙肝更经常被注意。结论：不管肝炎的病原学和活动，部件煽动性在肝渗入是类似的。全面煽动性的活动没与 HBsAg 和 HCVAg 的表示相关；然而， HBcAg 表示伴随了中等、严重的活动的长期的肝炎 B。