促凋亡因子PDCD5与Fas在肝癌及其癌旁组织中的表达

Elevated expression of PDCD5 and Fas in hepatocellular carcinoma and its adjacent tissues

目的:分析新凋亡促进因子-程序化细胞死亡分子5(programmed cell death 5,PDCD5)在肝细胞癌(hepatocellular carcinoma,HCC)发生发展中的作用.方法:用免疫组织化学法(immunohisto-chemistry,IHC)检测40例HCC及其癌旁组织(包括24例肝硬化和16例慢性肝炎)中的PDCD5和Fas蛋白表达.用等级资料Kruskal-WallisH检验方法进行统计学分析,并用Spearman's等级资料的相关分析比较PDCD5和Fas的表达差异.结果:PDCD5在肝癌组织中多呈阴性表达,在癌旁肝组织中表达增加.肝癌及其癌旁肝硬化肝炎各组间PDCD5表达具有显著性差异(χ2=46.03,P=0.000).Fas在肝癌组织及其癌旁肝硬化或慢性肝炎组织中的表达分布与PDCD5相似,各组间Fas表达有显著性差异(χ2=24.45P=0.000).PDCD5与Fas的相关性分析结果显示,两者呈正相关(r=0.839,P=0.001).结论:PDCD5是HCC发生、发展过程中的一个重要凋亡调控因子.

AIM：To investigate expression of programmed cell death 5 （PDCD5） and Fas in hepatocellular carcinoma （HCC） tissues and its adjacent tissues and liver cirrhosis tissues and thereby to explore relationship between PDCD5 and HCC. METHODS：Protein expression of PDCD5 and Fas from 40 HCC and its adjacent tissues （including 24 cases of liver cirrhosis, 16 cases ofchronic liver hepatitis） were analyzed using immunohistochemistry. Positive protein expression rates and intensity of PDCD5 and Fas were investigated under microscopy. Statistics were analyzed using Kruskal-Wallis H test and Spearman’s Rank correlation coeff icient. RESULTS：There was PDCD5 negative expression in HCC and raised expression rate in HCC adjacent tissues. There were significant difference in PDCD5 expression among HCC grpup, HCC adjacent tissue group and cirrhosis, chronic hepatitis group （χ^2 = 46.03, P = 0.000）. The same significance between HCC and cirrhosis or chronic hepatitis was detected for Fas expression （χ^2 = 24.45, P = 0.000）. Correlation analysis showed that PDCD5 was positively correlated with Fas （r = 0.839, P = 0.001）. CONCLUSION：PDCD5 is an important apoptosis-regulating factor in hepatocelluar carcinoma pathogenesis.