基于线粒体COⅡ和Cytb基因序列的8种漠甲系统发育关系

Phylogenetic analysis of eight species of Pimeliinae based on COⅡ and Cytb gene sequences.

对8种漠甲昆虫线粒体Cytb基因579bp和COⅡ基因585bp的序列片段进行联合分析。结果表明,8种甲虫在长度为1164bp的序列中碱基T,C,A,G的平均含量分别为33.7%,21.2%,33.5和11.6%,A+T平均含量明显高于G+C含量。密码子第三位点A+T含量高达77.2%,而该位点G的平均含量仅为4.0%。序列中碱基替换多发生于第三位点,转换略多于颠换,转换主要以C←T为主,颠换以A←T为主。以土甲族的Eumylada potanini为外群构建的系统发育树表明:漠王和漠甲以很高的置信值聚为一支,二者的关系与Bouchard最新的分类观点相吻合,即漠王族并入漠甲族成为一族;鳖甲族在支序图上另成一支,可视为一个单系群。

The phylogenetic relationships among 8 species of Pimeliinae based on a 579 bp Cytb and a 585 bp CO Ⅱ gene sequences were analyzed. The results showed that the average contents of T, C, A and G in the 1 164 bp sequences were 33.7%, 21.2%, 33.5 and 11.6%, respectively. The content of A ＋ T is significantly higher than that of C ＋ G, with 77.2% of A ＋ T at the third codon position but only 4.0% of G at the same position. Transition/ transversion mostly occur between C←→T or A←→T at the third position. With Eumylada potanini as outgroup, phylogenetie trees were reconstructed based on these DNA sequences. The trees showed that Platyopini and Pimeliini were placed in a elade, which is consistent with the current opinion that Platyopini was merged into Pimeliini. Meanwhile, Tentyriini formed another monophyletie elade in the tree.