激光穴位照射对类风湿性关节炎大鼠血清炎性介质、细胞因子及脑β-内啡肽的影响

The effect of laser irradiation on the level of histamine, 5-HT, IL-1 and TNF-α in serum and β-EP in brain tissue in rat with rheumatoid arthritis

目的探讨激光穴位照射对类风湿性关节炎模型大鼠血清组织胺（Hm）、5-羟色胺（5-HT）、白细胞介素-1（IL-1）、肿瘤坏死因子-α（TNF—α）及脑β-内啡肽（β—EP）的影响及其意义。方法将60只Wistar大鼠随机分为空白组、造模组、药物组和激光组，除空白组外，各组大鼠应用Freund’s完全佐剂制作关节炎模型。药物组采用药物治疗，激光组同时给予激光穴位照射及药物治疗。检测大鼠血清Hm、5-HT、IL-1、TNF—α及脑β—EP的含量。结果激光穴位照射有下调关节炎模型大鼠异常升高的血清Hm、5-HT、IL-1和TNF—α的作用，并可明显上调下丘脑内β—EP含量，疗效优于对照组（P〈0．05）。结论激光穴位照射可抑制血清炎性细胞因子，具有中枢镇痛作用，对类风湿性关节炎大鼠有较好的治疗效果。

Objective To observe the effect of laser irradiation on the level of histamine （Hm）, 5- hydroxytryptamine（5-HT） , interleukin-1 （1L-1）, tumor necrosis factor α （TNF-α）in serum and β- endorphin （β- EP）in brain tissue in rat model of rheumatoid arthritis. Methods A total of 60 Wistar rats were randomly divided into four groups：a normal group, a model group （ RA without treatment） , a laser ＋ drug treatment group （ RA treated by laser and drug） and a drug treatment group （RA treated by drug alone）. All the groups, except the normal one, were injected with complete Freund＇s adjuvant to make animal model of adjuvant arthritis. All the animals were then treated accordingly. The level of Hm, 5-HT, IL-1 and TNFα in serum and β-EP in brain tissue were measured. Results Laser downregulated the content of Hm, 5-HT, IL-1 and TNFα in serum and increased the content of β-EP in brain tissue. Conclusion Laser irradiation is an effective modality for treating the animal model of rheumatoid arthritis.