附着型硫酸盐还原菌的分离及其定量检测

Isolation and quantitative detection of adhesive sulfate reducing bacteria

针对附着型硫酸盐还原菌(A-SRB)对管道和罐壁的腐蚀问题,应用Hungate厌氧技术对附着型硫酸盐还原菌进行分离、系统发育分析和定量检测研究.研究表明,在地面系统中的SRB菌里,附着型SRB菌占多数,该菌株主要分布在厚壁菌门梭菌纲(Clostridia)、变形菌门的γ-变形菌纲(Gammaproteobacteria)和δ-变形菌纲(Deltaproteobacteria),其中梭菌属(Clostridium)为优势菌属,附着型的硫酸盐还原菌的种类比较新颖.通过安装在管壁上的检测装置和绝迹稀释法(MPN)联用实现附着型SRB菌的定量检测,该技术已在油田生产中实际应用.

Aiming at the corrosion on the inside wall of the pipeline and the pot due to the adhesive sulfate reducing bacteria （A -SRB）, the Hungate anaerobic technique was utilized to realize the isolation, 16S rDNA sequence phylogenetic analysis and quantitative detection on the adhesive sulfate reducing bacteria （A- SRB）. The study shows that the adhesive sulfate reducing bacteria （A -SRB） are the majority of the SRB in ground system. The strain is mainly distributed in Finmicutes Clostridia, Proteobacteria Gammaproteobacteria and Deltaproteobacteria. And the Clostridium species is the dominant species. The adhesive sulfate reducing bacteria （ A - SRB） are a novel species. The quantitative detection of the adhesive sulfate reducing bacteria （ A - SRB） was carried out by the detection device installed on the inside wall of the pipeline and the most probable number （MPN） method. And the technique has already been applied to the oilfield production.