去细胞光氧化处理牛颈静脉带瓣管道的形态学研究

Histomorphologic properties of bovine jugular vein conduit treated withdye-mediated photooxidation following decellularization

目的:评价去细胞结合光氧化技术处理的牛颈静脉带瓣管道(BJVC)组织相容性及组织稳定性。方法:取新鲜牛颈静脉20根,每根裁出4个带瓣血管片,随机分为4组,分别以染料介导光氧化(DMP)、戊二醛(GA)、去细胞(DC)及去细胞结合光氧化(DC+DMP)4种技术处理。通过组织蛋白提取实验观察BJVC去细胞后光氧化交联的时效性;建立大鼠皮下包埋模型,两月后获取组织标本,通过HE染色及电镜观察,比较4组试片的形态结构变化。结果:DMP交联的BJVC有明显的时效性,处理48h后交联程度至最大;与其他组比较,大鼠皮下包埋后去细胞结合光氧化组试片柔韧性好,纤维结构较完整,有轻度降解现象,细胞生长良好。结论:去细胞结合光氧化处理的BJVC组织相容性好,适于细胞浸润生长,组织稳定性较好,降解速率可通过DMP处理时间调节,是一种较理想的可降解带瓣血管支架材料。

Objective To determine the histocompatibility and histoconstancy of bovine jugular vein conduit（BJVC）treated by dye-mediated photooxidation following decellularization before and after implantation in Wistar rats.Methods Each of 20 fresh bovine jugular veins with a retained native valve procured from a slaughterhouse was cut into 4 trial patches with valves,which were randomly divided into 4 groups.The 4 groups were treated respectively by dye-mediated photooxidation（DMP）,glutaraldehyde（GA）,decellularization（DC）,and dye-mediated photooxidantion following decellularization（DC＋DMP）.One of the trial patches in each group was implanted subcutaneously in the same Wistar rat.Two months later,all trial rats were killed and the specimens were retrieved.Tissue protein extraction was used to estimate the cross-linked degree of BJVC treated by dye-mediated photooxidantion following decellularization.To observe the morphologic properties of the specimens,HE staining and electron microscopes were used.Results Compared with others,the patches in the DC＋DMP group were flexible,stretched,and relatively intact;lining endothelium was comparatively smooth;collagen fiber structure was slightly loose intact;and many cells were uniformly infiltrated in all layers.Conclusion BJVC treated by dye-mediated photooxidation following decellularization is superior to others in histocompatibility,and the rate of degradation can be regulated by the degree of dye-mediated photooxidation.