氨基胍对糖基化终产物干预后人肾系膜细胞MMP-2表达的影响

Effect of aminoguanidine on the matrix metalloproteinase-2 expression in AGEs-treated human renal mesangial cells

目的观察盐酸氨基胍(AG)对糖基化终产物(AGEs)干预后人肾系膜细胞(HRMC)基质金属蛋白酶2(MMP-2)表达的影响。方法运用糖基化终产物-牛血清白蛋白(AGE-BSA)干预HRMC,以及不同浓度AG单独或与AGE-BSA共同干预HRMC24小时;RT-PCR和Western blot分别检测HRMC MMP-2mRNA和蛋白表达。结果AGE-BSA明显降低HRMC MMP-2mRNA和蛋白表达(P<0.05),AG以浓度依赖的方式恢复AGE-BSA下调的HRMC MMP-2mRNA和蛋白表达(P<0.05)。结论AG可能通过拮抗AGEs对MMP-2表达的抑制效应而发挥其肾脏保护作用。

Objective To investigate the effect of aminoguanidine（AG）on the matrix metalloproteinase-2（MMP-2）expression in advanced glycosylation end products（AGEs）-treated human renal mesangial cells（HRMC）.Methods HRMCs were incubated with different concentrations of AG with or without AGE-BSA for 24 hours.The mRNA and protein expressions of MMP-2 in HRMC were analyzed by RT-PCR and Western blot,respectively.Results The expression levels of MMP-2 mRNA and protein in HRMC treated with AGE-BSA was significantly lower than that of control group（P〈0.05）.The depressed expressions of MMP-2 mRNA and protein in AGE-BSA-treated HRMC were increased by exposure to AG（P〈0.05）.Conclusions AG might play its reno-protection role via attenuation of AGEs-induced down-regulation of MMP-2.