uPA、MMP-3在退变椎间盘中的表达及意义

Expression and significance of uPA and MMP-3 in degenerative lumbar intervertebral disc

目的探讨尿激酶型纤溶酶原激活剂(uPA)、基质金属蛋白-3(MMP-3)在人类退变椎间盘组织中的表达变化。方法对照组为取自脊柱侧凸患者的正常腰椎间盘组织;实验组为腰椎间盘突出患者的退变椎间盘组织。分别进行免疫组织化学染色,比较2组椎间盘组织中uPA及MMP-3的光密度值。结果uPA和MMP-3在突出椎间盘组织中平均光密度值较正常椎间盘组织中的平均光密度值表达明显升高;相关分析显示突出椎间盘中uPA、MMP-3呈正相关关系。结论uPA和MMP-3均可能参与了人椎间盘组织的退变过程,且MMP-3与uPA在人类退变椎间盘组织中有相同的增高趋势。

Objective To explore the expression of uPA and MMP-3 in degenerative human intervertebral disc. Methods Sections of control group were from normal discs of patients with scoliosis, while those of experiment group were from degenerative discs of patients with intervertebral disk hernia. Both groups were immunostained with antibodies for uPA and MMP-3, and the optical density value of uPA and MMP-3 were compared between the 2 groups. Results Average optical density value of uPA and MMP-3 in degenerative discs were higher than that of normal discs. The expression of uPA and MMP-3 in the level of protein had positive correlation in cells of degeneration intervertabral disc. Condusion uPA and MMP- 3 in the cells of human intervertebral disc may be involved in the progress of human intervertebral disc degeneration and share the same increasing tendency.