摘要
目的观察抗转化生长因子-β2(TGF-β2)抗体对体外培养的滤过泡成纤维细胞Ⅰ、Ⅲ型胶原蛋白表达的影响。方法利用手术修复人眼小梁切除术后失败滤过泡修复时切除下来的瘢痕组织进行组织块培养,获得成纤维细胞(FB)。将0、0.01、0.1、1μg/mL抗TGF-β2抗体作用于FB24h以及0.14μg/mL(半数抑制率质量浓度,IC50)抗体作用0、24、48、72h,通过生物化学显色法、RT-PCR法检测抗TGF-β2抗体对成纤维细胞Ⅰ、Ⅲ型胶原蛋白的表达量。结果在FB中加入0、0.01、0.1、1μg/mL抗TGF-β2抗体作用24h及IC50质量浓度处理0、24、48、72h,结果显示随着抗TGF-β2抗体质量浓度增加及作用时间延长,Ⅰ、Ⅲ型胶原mRNA表达依次减少,羟脯氨酸(HPr)及胶原含量逐渐降低(P<0.05)。结论抗TGF-β2抗体能减少Ⅰ、Ⅲ型胶原蛋白的表达及合成。
Objective Scars of filtered bubbles are frequently to become the major reasons for filterable operations to fail. MMC and 5-Fu are the only drug used clinically against scars, but they have considerable side effect. The present study was to explore the influence of anti-TGF-β2antibody on the expression of former collagen Ⅰ and Ⅲ of filtered bubble fibroblasts cultured in vitro. Methods The scar tissue from human failed filtered bubbles during trabeculectomy was cultured primarily to obtain and passage the fibroblasts. 0,0.01,0.1 and 1 μg/mL of TGF-β2 antibody was added in cultured fibroblasts for 24 hours and 0.14 μg/mL of 50% inhibition rate（IC50） for 0,24,48 and 72 hours. The expression of TGF-β2 in former collagen Ⅰ and Ⅲ of fibroblasts was quantitatively detected using biochemical coloring and RT-PCR. Results After treated with 0,0. 01,0. 1 and 1 μg/mL of TGF-β2antibody for 24 hours and treated with IC50 for 0,24,48 and 72 hours,the band tensity of collagen Ⅰ and Ⅲ was gradually weaker with time prolone and the ratio of optical density integration was gradually reduced in a dose-dependent manner（ F = 16.75, P 〈 0.01 ）. In 0. 14 μg/mL anti-TGF-β2 group, the band and ratio of optical density integration followed a same model（ F = 17.12 ,P 〈0.01 ）. After treated with 0,0. 01,0. 1 and 1 μg/mL of TGF-β2antibody for 24 hours and 0. 14 μg/mL anti-TGF-β2 for 24,48 and 72 hours,the concentrations of HPr and collagen was significantly decreased （F = 18. 69,P 〈 0.01;F = 18.02,P 〈 0. 01 ）. Conclusion Anti-TGF-β2 antibody could reduce the expression and synthesis of former collagen Ⅰ and Ⅲ.
出处
《眼科研究》
CSCD
北大核心
2008年第7期508-511,共4页
Chinese Ophthalmic Research
基金
湛江市科技攻关项目资助(2006C01019)
