摘要
Wolbachia是一类广泛分布于节肢动物体内,可以引起节肢动物生殖行为改变的细胞内共生细菌。亚洲玉米螟Ostrinia furnacalis(Guen啨e)是我国常见的鳞翅目害虫,尤其对玉米危害严重。本文通过对Wolbahcia的wsp基因的PCR扩增及克隆测序,明确了Wolbachia在北京地区亚洲玉米螟野生种群中的分布情况。结果表明,在检测的种群中,存在两种类型的Wolbahcia,分别将其命名为wFurA(GenBank注册号为EU294311)和wFurB(GenBank注册号为EU294312);系统树分析表明其分别属于Wolbahcia A组和B组。本研究首次明确了在我国亚洲玉米螟的野生种群内存在着Wolbachia的超感染现象。
Wolbachia is a common and widespread group of bacteria found in reproductive tissues of many arthropods. Asian corn borer, Ostriniafurnacalis （Guenee）, is a common lepidopteran pest in China. In this study, two kinds of Wolbachia were detected in a natural population of O. furnacalis （Guenee） from Beijing based on the PCR and cloning of wsp gene. The wsp genes we sequenced were named as wFurA （GenBank accession no. EU294311） and wFurB （GenBank accession no. EU294312）, respectively. Phylogenetic tree analysis indicated that wFurA and wFurB belong to group A and group B of Wolbahcia, respectively. This is the first report of superinfection with distinct lineages of Wolbachia in wild O. furnacalis （Guenee） populations in China.
出处
《昆虫学报》
CAS
CSCD
北大核心
2008年第6期665-670,共6页
Acta Entomologica Sinica
基金
国家自然科学基金项目(30471166)
