补髓生血颗粒对慢性再生障碍性贫血患者血清酪氨酸激酶-3配体和纤维连接蛋白的影响

Effect of Busui Shengxue Granule on Serum Tyrosine Kinase 3 Ligand and Fibronectin in Patients with Chronic Aplastic Anemia

目的观察补髓生血颗粒治疗慢性再生障碍性贫血(CAA)的临床疗效及其对血清FMS样酪氨酸激酶-3配体(FMS-like tyrosine kinase3 ligand,FL)、纤维连接蛋白(fibronectin,Fn)表达水平的影响。方法将68例CAA患者分为两组,试验组35例应用补髓生血颗粒治疗,对照组33例使用再障生血片治疗,两组均治疗6个月。采用酶联免疫法检测患者外周血清FL和Fn治疗前后的表达水平。结果试验组和对照组治疗CAA的总有效率分别为74.3%(26/35例)和48.5%(16/33例),两组比较差异有统计学意义(P<0.05);且试验组阳虚型患者疗效〔93.8%(15/16例)〕优于肾阴虚型患者〔57.9%(11/19例)〕(P<0.05)。CAA患者外周血清FL和Fn均呈高表达状态,经治疗后两者均有一定程度下降,且试验组两项指标表达的调整程度皆优于对照组(P<0.05),而肾阳虚型患者血清FL和Fn表达水平较肾阴虚型患者更易得到纠正。结论补髓生血颗粒在临床疗效及改善CAA相关黏附分子FL和Fn表达水平均优于再障生血片,CAA阳虚型患者疗效优于阴虚型患者。

Objective To observe the clinical effect of Busui Shengxue Granule （BSG） in treating chronic aplastic anemia （CAA） and the changes of serum fibronectin （Fn） level and expression of FMS-like tyrosine kinase 3 ligand （FL）. Methods Sixty-eight patients with CAA were assigned to two groups. The 35 patients in the test group were treated with BSG and the 33 in the control group by Zaizhang Shengxue Tablet. Levels of Fn and FL were determined before and after 6-month treatment by enzyme linked immunosorbent assay. Results The clinical effective rate in the test group was 74. 3% （26/35 cases）, and that in the control group 48.5% （16/33 cases）, the difference between the two groups was significant （ P 〈 0. 05 ）. In the test group, patients of yang-deficiency type showed an effect superior to that of yin-deficiency type, 93.8% （15/16 cases） vs 57.9% （11/19 cases, P 〈 O. 05）. High expression manners of Fn and FL were shown in all CAA patients, the high expressions of both were lowered to some extent after treatment, but the improvement in the test group was better than that in the control group （ P 〈 0. 05 ）, and the deviation was more liable to be corrected in patients of Shen-yang deficiency type than that in patients of Shen-yin deficiency type. Conclusion BSG is better than Zaizhang Shengxue Tablet in clinical effect and improving the CAA related adhesive molecules, Fn and FL, suggesting that it is an effective remedy for preventing and treating CAA, with its curative effect more significant on patients of yang-deficiency type than that on patients of yin-deficiency type.