维持性血透患者血清体外诱导血管新生

In vitro induction of angiogenesis with sera medium of maintenance hemodialysis patients

目的:探讨维持性血液透析患者血清在体外诱导血管新生及其机制.方法:人脐静脉内皮细胞分别培养于100mL/L透析患者血清培养基(实验组)和100mL/L健康人血清培养基(对照组).倒置显微镜下观察血管样结构形成,MTT法检测细胞增殖,Transwell小室测定细胞迁徙能力,比色法检测细胞内活性氧(ROS)产生,Western Blot法检测p-p38蛋白表达.结果:实验组细胞培养30h平均血管样结构形成面积(1438±240)μm2,MTT平均吸光度值1.073±0.046,细胞小室平均迁移细胞数63.5±6.2,均高于对照组.实验组细胞培养10h p-p38表达及30h细胞内活性氧产生均明显增加.结论:维持性血透患者血清能够在体外诱导血管新生,p-p38,ROS表达增加可能是这种作用的细胞内机制.

AIM ： To explore the angiogenesis induced by uraemic sera medium in vitro and its mechanism. METHODS： Human unbilical vein endothelial cells （HUVECs） were respectively cultured in 100 mL/L haemodialysis patients sera medium （ experimental group） and 100 mL/L healthy persons sera medium （control group）. Vascular structure formation was observed under light microscope. Cell proliferation was examinded by MTT. Cell migration was measured in transwell chamber, ROS（ reactive oxygen species） was investigated by spectrophotometry and expression of p-p38 protein was evaluated by Western Blot. RESULTS： After HUVECs were treated for 30 hours in experimental group, the average vascular structure area was （1438 ±240） μm^2, mean optical absorption value was 1. 073 ± 0. 046 in MTT, and average number of migration cells was 63.7 ± 4.5, all of which were significantly different compared with control group. The expression of p-p38 protein and ROS increased respectively at 10 hours and 30 hours. CONCLUSION： Uraemic sera medium can induce angiogenesis in vitro, and the activation of p-p38 and ROS may be involved in the mechanism.