摘要
背景:中药骨康在临床上有明确的治疗骨质疏松的疗效。目的:假设中药骨康与雌激素同样具有调节成骨细胞的作用,观察其对成骨细胞胰岛素生长因子1表达的影响。设计、时间及地点:随机对照观察实验,体外细胞因子检测,于2005-09/2007-04在广州中医药大学附属骨伤科医院细胞培养实验室完成。材料:新生24h内的SD乳鼠,用于成骨细胞的分离培养。新西兰大白兔10只,用于制备药物血清。随机分为5组:空白对照组,中药骨康9.6g/kg、4.8g/kg、2.4g/kg含药血清组及雌二醇含药血清组,每组2只,雌雄各半。方法:各组大白兔按体表面积的方法给予中药骨康方的提取药物、雌激素和生理盐水灌胃,连续给药1周。最后一次用药后2h行心脏采血,分离血清。取第3代成骨细胞,经0.25%胰酶消化,将细胞悬液以终浓度1×107L-1接种到24孔培养板中,24h细胞贴壁后换为含10%各组血清的培养液,培养4d后收集培养上清液。主要观察指标:用酶联免疫吸附法检测培养上清液中胰岛素样生长因子1的含量。结果:中药骨康9.6g/kg、4.8g/kg组和雌二醇组胰岛素样生长因子1水平高于空白对照组(P<0.05),中药骨康9.6g/kg组和雌二醇组胰岛素样生长因子1水平高于中药骨康2.4g/kg组(P<0.05),中药骨康9.6g/kg组和雌二醇组之间比较差异无显著性(P>0.05),中药骨康2.4g/kg组与空白对照组比较差异无显著性(P>0.05)。结论:骨康用药量达到一定程度时,在促进成骨细胞生成胰岛素生长因子1方面的能力与雌二醇一致。
BACKGROUND: Chinese medicine Gukang has a clear effect of clinical treatment of osteoporosis.
OBJECTIVE: To investigate the effects of Chinese medicine Gukang on the expression of insulin-like growth factor 1 (IGF-1) in osteoblasts at the hypothesis of Chinese medicine Gukang possesses the regulating effect on osteoblasts identical with the estrogen.
DESIGN, TIME AND SETTING: A randomized control experiment of cytokine detection in vitro was performed at Cellular Culture Laboratory, Affiliated Orthopedic Hospital of Guangzhou Chinese Traditional Medicine University between September 2005 and April 2007.
MATERIALS: Ten SD neonatal rats were used for the separation and culture of osteoblasts, and ten New Zealand rabbits were used to prepare the drug serum and then were divided into 5 groups randomly: normal control group, high-, middle-, low-concentration groups and estradiol group. Each group contained two rabbits of either gender.
METHODS: Based on the surface area, the rabbits in the high-, middle-, and low-concentration groups were intragastrically administrated with 9.6, 4.8 and 2.4 g/kg serum containing Gukang. Estradiol group was intragastrically administrated with 15% estradiol solution, while the normal control group was given normal saline for l week. Two hours after the last administration, blood samples were taken from the heart of each rabbit. Then serum was collected. Osteoblasts at passage 3 were digested with 0.25% pancreatin, and the suspension at the final concentration of 1×10^7 L^-1 was inoculated into 24-well culture plates. Cellular attachment was observed 24 hours later, and meanwhile 10% serum containing corresponding drugs were added to the medium. The supernatant collection was performed 4 days later.
MAIN OUTCOME MEASURES: Enzyme-linked immunosorbent assay techniques were used to detect the expression of IGF-1 in supernatant.
RESULTS: Expression of IGF- 1 in osteoblasts in the serum containing the high and middle concentrations of Gukang and estradiol was increased compared with that in the normal control group (P 〈 0.05). The serum containing low concentration of Gukang was inferior to the high concentration of Gukang and the estradiol (P 〈 0.05). IGF- 1 expression was of insignificant differences between the high concentration group and the estradiol group, and between the low concentration group and the normal control group (P 〉 0.05).
CONCLUSION: The serum containing certain concentration of Chinese medicine Gukang can increase the secretion of IGF-1 in osteoblasts, and the effect is the same as that of estradiol.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第28期5414-5417,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research