应用^1H—MRS和LCModel研究鼻喷酒石酸布托啡诺对猪脑代谢物的影响及谷氨酸复合物的绝对定量

Absolute quantification of swine brain glutamate compounds concentration using MR spectroscopy and LCModel after nasal spraying butorphanol tartrate

目的通过氢质子磁共振波谱(1H-MRS)技术,研究鼻喷给药途径下中枢性镇痛药物对脑代谢物的影响,探讨鼻腔给药途径下1H-MRS及LCModel的应用价值。方法本实验选取9头健康2周龄乳猪(3.612±0.371)kg,行单体素PRESS序列扫描,获得鼻喷给药前后脑代谢物的波谱分析数据,用LCModel软件自动、客观地对波谱图进行线性拟合和基线校正。结果鼻喷酒石酸布托啡诺前1H-MRS测定动物脑谷氨酸复合物(Glx)浓度为(9.276±0.542)mmol/kg,鼻喷给药后Glx浓度为(7.283±0.540)mmol/kg。给药前后Glx浓度差异有统计学意义(t=2.826,P=0.022),而氮-乙酰天门冬氨酸(NAA)、胆碱(Cho)浓度改变无统计学意义,鼻喷给药前后NAA、Cho浓度分别为(6.094±0.384)mmol/kg∶(5.530±0.346)mmol/kg(t=1.270,P=0.240)和(1.547±0.114)mmol/kg∶(1.255±0.079)mmol/kg(t=1.800,P=0.110)。结论用1H-MRS技术及LCModel软件证明了Glx可能是中枢性止痛剂作用的重要神经递质。

Objective The purpose of this study was to investigate whether the central nervous system （CNS） analgesics affect the metaholites of swine brain by nasal spraying. Methods Single voxel proton magnetic resonance spectroscopy （^1H-MRS） was performed on 9 swine. The data were post-processed using LCModel software. Results Before nasal spraying butorphanol tartrate, the mean Glutamate compounds （GLxs） concentration was （9.276±0.542） mmol/kg. After nasal spraying, the mean Glx concentration was （7.283±0.540） mmol/kg. Using paired sample t-test we found that swine brain Glx level was significantly decreased after using nasal spray analgesic （t=2.826, P=0.022）. However, there were no significant differences in mean NAA and Choline （Cho） concentrations before and after nasal spraying[（6.094±0.384）mmol/kg vs. （5.530±0.346）mmol/kg （t=1.270,P=0.240） and （1.547±0.114） mmol/kg vs. （1.255±0.079） mmol/kg （t=1.800,P=0.110）, respectively）. Conclusion Our results imply that Glxs are nociceptive neurotransmitters and play an important role in the analgesia.