高血压患者血浆和淋巴细胞中血管紧张素Ⅱ、内皮素和一氧化氮的变化及关系

Changes in Plasmic and Lymphocytic AngiotensinⅡ, Endothelin and Nitric Oxide in Patients with Essential Hypertension

分析一组高血压病患者血浆和淋巴细胞中血管紧张素Ⅱ（ＡｎｇⅡ）、内皮素（ＥＴ）和一氧化氮（ＮＯ）含量的变化及它们间的相互关系。方法高血压（ＥＨ）组３９例，正常对照（ＮＣ）组４１例，放射免疫分析法测定ＡｎｇⅡ、ＥＴ，高效液相色谱分析法测定ＮＯ。结果ＥＨ患者血浆和淋巴细胞中ＥＴ均高于ＮＣ组（Ｐ分别＜０．０５和＜０．０１），ＮＯ低于ＮＣ组（Ｐ分别＜０．０５和＜０．０１）。除血浆ＥＴ和ＮＯ外，各指标改变ＥＨⅡ期较Ⅰ期明显（Ｐ分别＜０．０５和＜０．０１）。逐步回归分析表明，ＥＨ组平均动脉压（ＭＡＰ）与血浆及淋巴细胞中ＡｎｇⅡ、ＥＴ呈正相关，与ＮＯ负相关（ｒ分别＝０．６７，０．８１，Ｐ均＜０．０１）；ＥＨ组血浆和淋巴细胞中ＮＯ与ＡｎｇⅡ、ＥＴ均呈负相关（ｒ分别＝－０．６５１，－０．７２５，Ｐ均＜０．０１）。结论淋巴细胞反映血管内皮细胞内分泌功能比血浆更为敏感，与ＭＡＰ相关性更好，ＡｎｇⅡ、ＥＴ和ＮＯ三者分泌失平衡，是ＥＨ发病的重要原因之一。

Aim\ To examine the changes and the relationships of plasma and lymphocytic angiotensinⅡ(AngⅡ),endothelin(ET) and nitric oxide(NO) in essential hypertensive(EH) patients.\ Methods\ 39 EH patients and 41 nomotensive controls(NC) were studied. AngⅡ,ET were detected by radioimmunoassay and NO was measured by high performance liquid chromatograghy.\ Results\ The levels of plasma and lymphocyte AngⅡ, ET in EH group were higher than those in NC group(P<005 or P<001).\ The part alternate in stage Ⅱ EH were more obvious then those in stage Ⅰ.\ Multiple progressive regression analysis indicated that MAP has a positively correlated with AngⅡand ET, but negative correlated with NO(plasma: r=067;lymphocytes: r=081 P<001).\ In EH groups, NO has negative correlation with AngⅡand ET(plasma: r=-0651; lymphocytes: r=-0725, P<001).\ ConclusionLymphocytes could be used as an indirect method to estimate the endocrine function of endothelium cells. It is more sensitive and has a better correlation with MAP than those in plasma. The imbalance of these biological substances may contributes to hypertensive pathogenesis.