低氧诱导因子-1α基因C958G单核苷酸多态性与有氧运动能力关联性研究

Association between SNP C958G in Hypoxia-inducible Factor Gene and Aerobic Capacity

目的:选取HIF-1α基因第7内含子上的C958G单核苷酸多态(SNP/C958G)解析,并与有氧耐力训练后生理表型指标进行关联性研究,探讨该多态位点作为预测有氧耐力训练效果的分子遗传学标记的可行性。方法:实验对象为102名中国北方士兵。受试者进行18周有氧耐力训练,测试有氧耐力训练前后血红蛋白(Hb)、最大耗氧量(VO_2max)及通气无氧阈(VO_2VT)等指标,应用PCR-RFLP解析HIF-1α基因SNP/C958G的基因型,采用关联(Association-Study)研究方法,研究18周有氧耐力训练前Hb、VO_2max及VO_2VT等指标变化量与HIF-1α基因SNP/C958G关联性。结果:HIF-1α基因SNP/C958G基因型在士兵中分布频率经卡方检验[x^2(df=2)=0.20,P>0.05]符合Hardy-Weinberg平衡。SNP/C958G的GG基因型受试者18周有氧耐力训练后Hb、VO_2 max、VT/VO_2分别提高了5%、5%、4%,同时,GG基因型与CC、CG基因型受试者有氧耐力训练后Hb、VO_2max、VT/VO_2的变化量相比明显增加(P<0.05)。结论:HIF-1α基因SNP/C958G多态性GG基因型可作为预测有氧耐力训后Hb、VO_2max、VT/VO_2敏感性变化的分子遗传学标记。

Objective： In present study, we researched the association between single nucleotide polymorphism （SNP） C958G,which located in intron 7 of HIF-α gene, and aerobic capacity, and explored feasibility that SNP C958G was as genetic mark for predicting the effect of aerobic endurance training. Method： The 102 soldiers selected from north region of China who performed aerobic training program for 18 weeks. Some phenotype parameters, such as hemoglobin （Hb）, maximal oxygen uptake （VO2 max）, ventilation threshold （VT/VO2）, were measured before and after aerobic training. Meantime, Genotyping of SNP C958G of all subjects was performed by polymerase chain reaction amplification. Results：Departures from Hardy-weinberg equilibrium were not statistically significant in SNP C958G. After 18 weeks aerobic training,The Hb,VO2max, VO2VT increased separately by 5%.5%.4% in subjects with GG genotype. And the increase of Hb, VO2 max, VT/VO2 of subjects with GG genotype were significant greater than those of the CC and CG genotype after aerobic training （P〈0.05）. Conclusion： The results suggested that SNP/C958G in HIF-1α gene was a genetic factor that affected the responses to aerobic training.