葛根素对自发性高血压大鼠肾血管核因子-κB表达的影响

The Effects of Puerarin on Renal Arterial NF-κB Expression in Spontaneous Hypertensive Rats

目的探讨葛根素对自发性高血压大鼠核因子-kappaB(NF-κB)表达的影响。方法将24只自发性高血压大鼠随机分为对照组、葛根素组(腹腔注射葛根素)和贝那普利组(灌胃贝那普利)。免疫组化染色法观察各组肾血管NF-κB的表达,放免法检测各组大鼠血清血管紧张素Ⅱ;光镜下观察各组大鼠肾动脉狭窄的程度。结果NF-κB在自发性高血压大鼠肾血管均有表达;与对照组比较,葛根素组和贝那普利组NF-κB表达降低,血管紧张素Ⅱ下降,肾动脉狭窄程度明显减轻。结论葛根素可能通过抑制血管紧张素Ⅱ引起的NF-κB活化以下调NF-κB表达,从而减轻肾动脉狭窄程度。

Objective： To explore the effects of Puerarin on NF-κB expression in kidneys of spontaneous hypertensive rats. Methods： 24 spontaneous hypertensive rats（SHR） were divided into three groups; control group, Purearin group（intraperitoneal injection） and benazepril group （intragastric administration） . Renal NF -κB expression in SHR were studied by immunocyto chemical techniques, angiotension Ⅱ in every group rat＇ s serum were assayed by radiation immunological test. Renal artery stricture of every group rat were obseryed under optic microscope. Results： NF -κB was expressed in spontaneous hypertensive rats; as compared with control group, NF -κBexpression decreased remarkably in benazepril group and Puerarin group. And as compared with control group, angiotension Ⅱ expression was downregulated in benazepril gorup and Puerarin group with statistical difference, and renal artery stricture in the two groups was alleviateded distinctly. Conclusion： Puerarin alleviateds renal artery stricture by the way it inhibits angiotension Ⅱ expression which actives NF -κB.