黄连丁香提取物在模拟胃液酸性条件下对亚硝胺生成的影响

Influence of Extracts of Rhizoma Coptidis and Flos Caryophylli on the Production of Diethylnitrosamine in Vitro

阻断或减少亚硝胺的生成是预防肿瘤发生的一个重要措施。本实验在试管内模拟胃液酸性环境，利用高效液相色谱技术进行二乙基亚硝胺生成的实验研究，并观察丁香、黄连提取物对合成的影响。结果表明，在该实验条件下亚硝酸钠与二乙胺反应，合成物与二乙基亚硝胺标准品的保留时间基本一致，所测得的生成物生成率为２４．５％。试验结果还表明黄连生物碱可引起生成物含量减少。

nhibiting or reducing the production of diethylnitrosamine is an important measure for the prevention of oncogenesis. In this report, the production of diethylnitrosamine under minetic gastric acid condition in vitro and the effect of Rhizoma Coptidis and Flos Caryophylli extracts on its production was observed with HPLC. The results showed that diethylamine reacts with sodium nitrite and the retention time of its product is the same as that of the standardized product of diethylnitrosamine. Its synthetic rate was 24.5% and could be reduced by alkaloid of Rhizoma Coptidis.