血管内皮生长因子和血小板衍化内皮生长因子的表达在甲胎蛋白阴性肝细胞癌根治性切除术后预后预测中的作用

Tissue microarray analysis of expression of vascular endothelial growth factor and platelet-derived endothelial cell growth factor in α-fetoprotein-negative hepatocellular carcinoma and its relationship with prognosis after radical resection

目的探讨血管内皮生长因子（VEGF）和血小板衍化内皮生长因子（PD-ECGF）在甲胎蛋白（AFP）阴性肝细胞癌切除术后预后预测中的作用。方法利用组织微阵列技术（tissue microarray）和免疫组织化学方法回顾性研究200例AFP阴性肝癌患者肿瘤中VEGF及PD-ECGF的表达情况，分析其与肝细胞癌切除术后预后的关系。结果VEGF及PD-ECGF表达阳性组与相应阴性组患者之间的生存率和无瘤生存率差异无统计学意义（P〉0．05），而VEGF及PD-ECGF表达阳性组的术后复发率比相应阴性组显著增高（P〈0．05）。VEGF和PD-ECGF同时阳性表达组其复发率更显著高于同时阴性组（P〈0．01），其生存率和无瘤生存率亦显著降低（P〈0．05）。结论对于甲胎蛋白阴性肝癌患者，VEGF和PD-ECGF同时表达阳性提示术后易发生复发，预后不佳。

Objective To study the expression of vascular endothelial growth factor （VEGF） and platelet-derived endothelial cell growth factor （PD-ECGF） and its relationship with the prognosis after radical resection for α-fetoprotein （AFP）-negative hepatocellular carcinoma （HCC）. Methods The expression of VEGF and PD-ECGF in 200 patients with AFP-negative HCC proven by surgery and pathology during Jan. 1997 to Dec. 2000 was analyzed retrospectively by high-throughput tissue microarray technology and immunohistochemistry. Kaplan-Meier method,log-rank test and chi-square test were used to analyze the relationship between the expression of the two angiogenetic factors with the prognosis of AFP-negative HCC. Results Compared with VEGF-positive group and PD-ECGF-positive group, the patients＇ survival rate and tumor-free survival rate rather than recurrence rate had no significant difference in the negativeexpression group, respectively. The recurrence rate was also higher in the both positive groups than in the both negative groups. Moreover, the patients in both positive groups had a significantly low survival and tumor-free survival rate. Conclusion Positive expression of both VEGF and PD-ECGF indicated high risk of tumor recurrence and a poor prognosis after radical resection for AFP-negative HCC.