复方黄连胶囊对大鼠糖尿病肾病及血液流变学异常的干预作用

Effect of Compound Rhizoma Coptidis Capsula on diabetic nephropathy and hemorrheological abnormalities in rats

目的探讨复方黄连胶囊(CRCC)对大鼠糖尿病肾病(DN)及血液流变学异常的干预作用。方法以链脲佐菌素(STZ)复制早期DN大鼠模型,动物分为对照组、模型组、3个CRCC(1.09、2.18、4.36g/kg)治疗组、消渴丸治疗组,30d后检测空腹血糖(FBG)、尿素氮(BUN)、肌酐(Scr)、胰岛素(INS)、尿蛋白(Upro)及血液流变学;光镜和电镜观察肾组织形态学和超微结构的改变。结果与模型组比较,CRCC治疗组糖尿病大鼠FBG、BUN、Scr及Upro水平降低,INS升高;全血黏度、血浆黏度、红细胞聚集指数(AI)、红细胞刚性指数(IR)和纤维蛋白原的量(fib)降低,红细胞压积(HCT)和红细胞变形指数(IED)无变化;肾组织形态学异常和超微结构病变改善。结论CRCC对早期DN大鼠微血管病变具有明显的干预作用,延缓慢性病理进展,但不能阻断DN,其机制可能与改善血液流变学有关。

Objective To explore the effect of Compound Rhizoma Coptidis Capsula （CRCC） on early diabetic nephropathy （DN） and hemorrheological abnormalities in rats. Methods The rat model of DN was induced by injection of streptozotocin （STZ）. The rats were divided into six groups： normal group, model group, three CRCC treatment groups, and Xiaoke Wan （XKW） treatment group. The fasting blood glucose （FBG）, blood urea nitrogen （BUN）, serum creatinine （Scr）, insulin （INS）, urinary protein （Upro）, and hemorrheology were tested; The pathological changes and ultrastructures in renal tissue of diabetic rats were observed by optical and electron micrography in diabetic rats 30 d after the treatment. Results Compared to model group, CRCC could reduce the levels of FBG, BUN, Scr in blood, and Upro in 24-hour urine, and increase the level of INS in blood, and reduce blood and plasma viscosity, red cell aggregation index （AI）, index of rigidity （IR）, and fibrinogen level （fib）, but it could not significantly alter hematocrit （HCT） and index of erythrocyte deformability （IED）. CRCC could ameliorate the pathological changes and ultrastructureal lesion in renal tissue of DN rats. Conclusion CRCC could alleviate the disorder of glucose in blood, improve the renal function, and slow down the progression of microvascular complications in DN rats. The mechanism is probable by the intervention in hemorrheological abnormalities.