裕丹参愈伤组织诱导、继代及植株再生的研究

Inducement,subculture,and plantlets regeneration of callus from Salvia miltiorrhiza

目的建立裕丹参愈伤组织的诱导、继代及植株再生体系。方法比较不同的外植体基本培养基及植物生长调节剂对裕丹参愈伤组织的诱导、继代及植株再生的影响。结果MS+6-BA2mg/L+NAA0.2～2mg/L有利于叶柄和叶片愈伤组织的诱导,其出愈率均为100%,但叶柄出愈量更大;MS+6-BA2mg/L有利于叶片芽的分化,其分化率达55.6%,并有直接成芽现象;B5+6-BA1mg/L+2,4-D1mg/L是愈伤组织继代培养的最佳培养基。结论愈伤组织诱导的最佳外植体为叶柄,最佳培养基为MS+6-BA2mg/L+NAA0.2～2mg/L;诱导芽分化的最佳外植体为叶片,最佳培养基为MS+6-BA2mg/L;愈伤组织继代培养的最佳培养基为B5+6-BA1mg/L+2,4-D1mg/L。

Objective To establish the system of inducement, subculture, and plantlets regeneration of callus from Salvia rniltiorrhiza. Methods Compared with the effect of different explants, basic media, and plant growth regulator on the inducement, subculture of callus, and the differentiation of buds. Results MS＋6-BA 2 mg/L-＋-NAA 0.2-2 mg/L was propitious to the inducement of callus and the ratio of induced callus, for both the leafstalk and lamina, was 100%, but quantity of induced callus was more of The ratio of buds differentiation was 55.6% on the medium of MS＋6-BA 2 rag/L, and it could grown into buds directly. B5＋6-BA 1 mg/L＋2, 4-D 1 mg/L was the better subculture medium of callus. Conclusion So for the callus inducement, the better explant and medium are leafstalk and MS＋6-BA 2 mg/L＋NAA 0.2-2 mg/L, respectively; For the buds differentiation, the better explant and medium are lamina and MS-＋6-BA 2 mg/L, respectively; For the subculture of callus, B5＋6-BA 1 mg/L＋2, 4-D 1 mg/L is better.