果桑侧芽生长点组织快繁初探

The Primary Study of Mulberry Rapid Propagation by Culture of Side Bud Tissue

果桑(Morus spp)具有很高的经济价值,是目前中国农业产业结构调整的优选树种。桑苗可通过组织培养快速繁殖。笔者以果桑侧芽生长点为材料,研究各种消毒条件、不同浓度的培养基和不同褐化抑制剂对其组织快繁的影响。实验结果得到了生长茁壮的愈伤组织,并诱导生根。主要结果如下:果桑生长点先用75%酒精消毒30s,再用加入少量吐温-80的0.1%HgCl2消毒8min,在MS+6-BA2mg/L+IAA0.5mg/L的培养基上诱导愈伤率可达80%。在生根培养基MS+IAA0.1mg/L+6-BA0.5mg/L上,生根率可达70%。如果直接进行不定芽诱导,在MS+IAA0.2mg/L+6-BA3.0mg/L的培养基上,每个茎段平均可以产生3个不定芽。抗坏血酸可以在一定程度上抑制愈伤组织的褐化现象。但是在整个果桑组织培养过程中仍然存在很多问题,需要进一步研究。

Mulberry （Morus spp） is the optimized species in agricultural structure adjustment of China and has a high economic value. The seedling of mulberry can be propagated rapidly by tissue culture. The author focused on the effects of various sterilization conditions, different concentration of mediums and different browning inhibition on tissue rapid propagation using Mulberry＇s side bud tissue as explants. As a result, the explants have grown into healthy and strong callus and been inducted into roots. The main results were as follows： The Mulberry＇s side bud tissue was disinfected for 30 seconds using 75% alcohol firstly, then for 8 minutes by HgC12 solution with a few of Tween-80. In the culture medium of MS＋6-BA 2mg/L ＋ IAA 0.5mg/ L, the induction rate of callus was about 80%. In the rooting culture medium of MS ＋ IAA 0.1mg/L ＋ 6 - BA 0.5mg/L, the rooting rate was about 70%. If taking adventitious bud induction directly, each stem can produce three adventitious bud averagely in the culture medium of MS ＋IAA 0.2mg/L ＋ 6 - BA 3.0mg/L. Ascorbic acid can inhibit callus browning to a certain extent. But there are still many problems in the process of Mulberry＇s tissue rapid propagation and further study is necessary.