大白菜亲缘关系研究

Studies on Genetic Relationships among Chinese Cabbage(Brassica campestris L.ssp.Pekinensis)

为更有效地利用现有资源、挖掘优异基因为育种和生产服务,对大白菜种质资源进行亲缘关系研究在理论和应用上都具有重要意义。本研究利用6对AFLP引物对89份来源和特性不同的大白菜(Brassica campestrisL.ssp.pekinensis)种质进行系统评价和分类研究。6对AFLP引物共扩增出286条带,123条为多态性带,占总数的43.01%。不同引物组合扩增出的总带数和多态性带数差异较大,其中,引物组合E-ACT/M-CAC的扩增效果最好,多态性条带最多,占总带数的54.35%。聚类分析显示:中国大白菜种质资源遗传多样性丰富,参试种质间最大遗传距离为0.359,遗传背景广泛。以0.73的相似系数为阈值,所有材料被分为四大组群,其中浙江黄芽菜单独构成一个组群,明显地区分于其他大多数大白菜品种("主体"组群)。在"主体"组群中,参试的台湾种质和大陆种质分为两个亚组,日本和韩国种质均被聚到了大陆种质亚组,表明供试的日本和韩国材料与中国大陆种质遗传亲缘关系密切。

It is meaningful to study the genetic relationships among Chinese cabbages for potentializing gene resources and serving the breeding. In this study, 89 accessions of Chinese cabbage （Brassica campestris L. ssp. pekinensis） germplasm were employed to study the genetic relationships among Chinese cabbages using AFLP makers. 6 pairs of primer generated 286 bands, including 123 （43.01%） polymorphic bands. There is obvious difference among the numbers of total bands and polymorphic bands produced by different pairs of primer. E-ACT/M-CAC showed the best amplification effect and generated plentiful polymorphic bands that occupying 54.35% of total. Based on the AFLP markers, clustering analysis of AFLP showed that Chinese cabbage possesses broad variation with utmost genetic distance of 0.359. All the materials were divided into 4 groups with the threshold of 0.73 of similarity coefficient. ＂Zhejiang huangyacai＂ was separated from the group of most of Chinese cabbage and formed into another independent group. The result indicated that most of Chinese cabbage and the lines from Japan and Korea which were more genetically similar to the mainland materials formed into one group. In this group the lines of Taiwan germplasms formed into an independent subgroup, and the materials in most parts of mainland formed into another subgroup.