摘要
目的探讨弥漫性脑损伤脑组织不同时间段iNOSmRNA表达及与脑组织水肿的关系。方法依据Marmarou's弥漫性脑损伤动物模型有改进,应用SD雄性大鼠55只,随机分为两组:假手术(对照组)(n=5)和弥漫性脑损伤组(n=50),损伤组再按照不同时间段分组(n=5),损伤后大鼠自由进食饮水,按0.5h、1h、3h、6h、12h、24h、48h、72h、1w、2w等时间段处死大鼠,提取大鼠皮层脑组织,一部分脑组织应用realtime RT-PCR检测对照组与不同时间段外伤组iNOSmRNA表达,另取一部分脑组织蘸干脑组织血迹,应用分析天平测定脑组织湿重,放入C101型电热恒温鼓风干燥箱105℃烘干至恒重,应用分析天平测定脑组织干重,应用脑组织干湿重比表示脑组织含水量。结果对照组,0.5h、1h、3h、6h、12h、24h、48h、72h、1w、2w时间段外伤组iNOSmRNACt值分别为:(34.999±0.444),(35.931±0.409),(40.376±0.551),(41.263±0.755),(42.539±0.394),(44.325±0.171),(44.165±0.255),(41.514±0.374),(41.390±0.158),(41.317±0.245),(35.204±0.504)。用kurskal-wallis秩和检验P<0.05,对照组不同时间组之间差别有统计学意义;脑组织含水量分别为:(0.78118±1.0532)E,(0.78505±1.6969)E,(0.79998±9.7477)E,(0.80231±3.4466)E,(0.80342±8.5095)E,(0.80913±1.0123)E,(0.80455±3.7740)E,(0.80196±5.5710)E,(0.80022±5.6378)E,(0.79998±5.2919)E,(0.78505±1.6969)E。用kurskal-wallis秩和检验P<0.01,对照组不同时间组之间含水量差别有统计学意义。结论①弥漫性脑损伤后脑组织iNOSmRNA表达早期有升高趋势,12h达到高峰,24h仍然处于高水平状态,随后下降,持续时间长达1周。②弥漫性脑损伤后脑组织含水量增加,6h增幅快,12h到达较高水平,持续两周。③iNOSmRNA表达可能是脑组织含水量增加的一个原因,且可能是脑组织含水量增高长期性的一个原因。
Objective To determine and evaluate the transformation of the edema and the expression of iNOSmRNA in brain following diffuse brain injury (DBI). Methods Fifty -five male SD rats were randomly divided into two groups: control ( n = 5 ), DBI (they were divided into ten groups again, n = 5). Diffuse contusion and laceration of brain was produced by Marmarou's brain injury model. Determine and evaluate the water contained in brain (edema of brain) and the expression of iNOSmRNA by realtime RT - PCR at different time following diffuse brain injury. Results The expression of iNOSmRNA upregulates rapidly following diffuse brain injury. The expression of iNOSmRNA gets to top at 12 hours. The expression of iNOSmRNA are different among groups by karskal - wallis test, P 〈 0.05. The water contained in damaged brain increase rapidly in 6 hours. The water contained in damaged brain are different among groups by kurskal - wallis test, P 〈 0.05. It gets to maximum at 12 hours. Conclusion (1) the expression of iNOSmRNA upregulates rapidly following diffuse brain injury. It gets to maximum at 12 hours. (2)The water contained in damaged brain increase rapidly at short time. It can reach higher leval at 6 hours. (3)the expression of iNOSmRNA following diffuse brain injury may be the cause that the water contained in damaged brain increase rapidly.
出处
《医药论坛杂志》
2008年第14期1-3,8,共4页
Journal of Medical Forum