空间环境对恶性黑色素瘤血管生成拟态的影响

Vasculogenic Mimicry of Melanoma B16 Cell Clones Mutagenized by Outer Space Environment

观察空间诱变后恶性黑色素瘤B16细胞株的在小鼠体内肿瘤组织中血管生成拟态数目的改变,并探讨可能的机理。应用细胞低温长期生存系统,将B16细胞株搭载于中国第20号返回式卫星,返地后单克隆化,从得到的110株单克隆空间诱变B16细胞株中随机选取7株,编号为38#～44#,常规培养6代后和对照细胞株在荧光倒置显微镜下观察肿瘤细胞的形态;同时采用MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,又称噻唑蓝)法,检测细胞增殖情况,将增殖差异较大的39#和44#细胞株接皮下种于C57BL/6J小鼠,两周后颈椎脱臼法处死小鼠,取出瘤体,经福尔马林固定后,采用CD31和PAS套染的方法观测肿瘤组织血管生成拟态的情况,并推测其与肿瘤生长速度和转移可能关系。

To study the number of vasculogenic mimicry of melanoma B16 cell clones mutagenized by outer space environment and the possible mechanism, B16 cells were carried by the Chinese 20th recoverable satellite to the outer space, and were harvested after 18 days＇ spaceflight and then monocloned. Seven cell clones numbered 38#～44#, which were randomly selected from the total 110 clones obtained, and the control clones were routinely cultured. After six generations the cultured cells were experimented with inverted microscope and MTT assay, a method for analysis of cell growth characteristics. Two clones were selected to be remarkably different from the control clone in morphology and growth speed. Cultured melanoma B16 cells in vitro might be mutated by outer space environment. It is impossible to infer the growth velocity and metabasis of B16 cell clones in favor of mankind.