诺瓦克病毒抗原检测在急性胃肠炎病原学诊断中的应用

Application of Norwalk Virus Antigen Detection in Etiological Diagnosis of Acute Gastroenteritis

目的为急性胃肠炎病原学诊断和预防控制提供科学依据。方法采用酶联免疫吸附试验(ELISA)法在急性胃肠炎患者粪便中查找诺瓦克病毒(NV)抗原,进行急性胃肠炎病原学诊断。结果2005年6月—2007年6月在梅州市6起群体性胃肠炎事件中,证实其中3起是由NV引起。结论采用ELISA法在急性胃肠炎患者粪便中查找NV抗原,方法灵敏度高,诊断快速,且较经济,在急性胃肠炎病原学诊断中具有重要的价值。

[ Objective] To provide the scientific evidence for acute gastroenteritis etiological diagnosis and its control and prevention. [ Methods] ELISA was applied to determine Norwalk virus （NV） antigen in the stool samples of acute gastroenteritis patients for etiological diagnosis of acute gastroenteritis. [ Results] Six groups of acute gastroenteritis cases were reported from June 2005 to June 2007 in Meizhou city, three of them were caused by Norwalk virus. [ Conclusion] ELISA was applied to determine NV antigen in the stool samples of acute gastroenteritis patients, for its high sensitivity, rapid diagnosis and more economical characteristics： It has important practical value in the etiological diagnosis of acute gastroenteritis.