人工合成小麦新种质抗条锈性鉴定与Yr18基因检测

Identification of resistance to stripe rust in synthetic wheat germplasms and detection of Yr18 gene

对97份来自CIMMYT的人工合成小麦新种质进行了主要农艺性状考察、Yr18分子检测和成株期田间抗锈性接种鉴定。结果表明:参鉴种质间主要农艺性状变异程度较大,尤其千粒重普遍偏高,有30份材料千粒重达50 g以上、占30.9%;利用csLV34标记检测到合成20和合成43携带Yr18,占2.1%;鉴选出成株期呈抗性反应的材料30份、占30.9%,高度慢条锈材料19份、占19.6%,其中合成2、5、14、60、75、76、78、82、83、84计10个合成种同时表现高抗和高度慢锈。这些大粒、抗条锈或慢条锈合成小麦种质的鉴定筛选,为选育小麦新品种提供了优异资源。

Main agronomic traits of 97 synthetic wheat germplasms from CIMMYT were investigated. Moreover, Yr18 gene was detected with csLV34 and resistance to stripe rust was identified at adult stage. The results indicated that there were major differences in main agronomic characteristics among tested materials, especially 1 000-kernelweight, which increased in most cases, with 30 novel lines exceeding 50 grams and accounting for 30.9 percent. Two materials, namely synthetic wheat No. 20 and No. 43 with Yr18, were found by the marker csLV34 and the occurrence frequency was 2.1%. There were 30 lines with resistance to stripe rust, 19 lines with highly slow-rusting resistance, 10 lines, including synthetic wheat No. 2, No. 5, No. 14, No. 60, No. 75, No. 76, No. 78, No. 82, No. 83 and No. 84, had high resistance and high slow-rusting resistance to stripe rust. Selection of big grains and slow rusting germplasms will offer excellent resources for common wheat breeding.