反义基因逆转肿瘤细胞多药耐药诱导细胞凋亡的研究

Study of antisense oligodeoxynucleotide in reversing multidrug resistance and inducing apoptosis of tumor cells

目的：探讨克服肿瘤细胞多药耐药（ＭＤＲ）的方法，提高化疗效果。方法：采用多药耐药反义基因（ｍｄｒ－１－ＡＳＰＳ－ＯＤＮ）逆转Ｋ５６２／ＡＤＭ肿瘤细胞的ＭＤＲ，而诱导肿瘤细胞凋亡。结果：ｍｄｒ－１－ＡＳＰＳ－ＯＤＮ诱导Ｋ５６２／ＡＤＭ细胞产生大量ＤＮＡ断片，流式细胞仪检测发现几乎全部ｍｄｒ－１＋Ｋ５６２／ＡＤＭ细胞发生凋亡。结论：ｍｄｒ－１－ＡＳＰＳ－ＯＤＮ能有效、特异地抑制ｍｄｒ－１基因表达，逆转肿瘤细胞的ＭＤＲ，促进阿霉素诱导Ｋ５６２／ＡＤＭ细胞凋亡，为其临床应用提供理论依据。

Objective:To explore an approach to the reversal of multidrug resistance(MDR) of tumor cells.Methods:Antisense oligodeoxynucleotide(mdr1AS PSODN) was used.Results:A large amount of DNA fragments was found in mdr1AS PSODN treated K562/ADM cells and almost all the mdr1+ K562/ADM cells underwent apoptosis.Conclusion: mdr1AS PSODN could inhibit specifically mdr1 gene expression,effectively reverse MDR of the tumor cells and promote ADM induced apoptosis of mdr1+ K562/ADM.