摘要
目的了解宁夏流行风疹病毒的分子生物学特征,确定其基因型别。方法用Vero-Slam细胞从风疹患者的标本中分离风疹病毒,通过逆转录-聚合酶链反应(RT-PCR)和序列测定与分析鉴定其基因型。结果宁夏首次分离的6株风疹病毒均为1E基因型,与2株WHO风疹病毒1E基因型参考株(M1-MAL-01,T14-CH-02)核苷酸同源性分别为97.6%、98.7%-98.9%,氨基酸同源性分别为99.5%、100%。结论1E基因型风疹病毒在宁夏存在着流行。
Objective To understand genetic characteristics and genotype of rubella virus circulated in Ningxia. Methods Rubella viruses were isolated from specimens of patients on Veto -Slam cells ,reverse- transcription polymerase chain reaction（ RT -PCR） and sequencing were performed to identify the genotypes. Results 6 strains of rubella virus firstly isolated in Ningxia were genotype 1E. By comparision with 2 other genotype IE reference isolates （M1 - MAL -01, T14 - CH -02 ）, the identities of nucleotides were 97. 6% ,98.7% - 98.9% respectively,and the identities of amino acids were 99.5% ,100% respectively. Conclusion Genotype 1E rubella viruses circulated in Ningxia.
出处
《宁夏医学杂志》
CAS
2008年第7期577-579,共3页
Ningxia Medical Journal
关键词
风疹病毒
序列分析
基因型
Rubella virus
Sequence analysis
Genotype
