制备工艺对柴苓汤颗粒抗大鼠慢性肾功能衰竭的影响研究

Curative Effects of Chailing Decoction Granules Prepared by Different Preparation Technology for Chronic Renal Failure in Rats

目的:考察不同工艺制备的柴苓汤颗粒抗大鼠慢性肾功能衰竭(CRF)的效果。方法:给药组与模型对照组大鼠用腺嘌呤诱发CRF,给药组分别给予日本津村、仿日本工艺与习用工艺颗粒,模型对照组与健康对照组给予生理盐水,观察尿、血生化指标以及肾脏病理学形态变化。结果:与模型对照组比较,日本津村组、仿日本工艺组的血尿素氮、血总蛋白、血胆固醇、尿蛋白含量与肾间质损伤程度均显著降低(P<0.01或P<0.05);习用工艺组仅在20倍剂量时血尿素氮、尿蛋白含量与炎细胞浸润程度显著降低(P<0.01或P<0.05)。结论:津村与仿日本工艺柴苓汤颗粒对CRF大鼠的治疗作用优于习用工艺柴苓汤颗粒。

OBJECTIVE： To study the curative effect of different Chailing decoction granules on chronic renal failure （CRF） in rats. METHODS： SD rats in trial groups and model group were given adenine to induce CRF model. The trial groups were treated with Japanese Tsumura granules, imitating Japanese method granules or Chinese normal method granule. The model group and the control group were treated with saline. The urine, blood biochemical parameters as well as morphological change of kidney were monitored. RESULTS： Compared with model group, Japanese Tsumura granules and imitating Japanese method granules significantly reduced the levels of blood ureanitrogen, serum total protein,serum cholesterol and urinary protein,and the damage on renal i nterstitium was lessened significantly（P 〈 0.01, P 〈 0.05） .Chinese normal method granules at 20 times doses could significantly lower levels of blood ureanitrogen, urinary protein and lessen the degree of inflammatory cell infiltration （P 〈 0.01, P〈0.05） . CONCLUSION： The Japanese Tsumura and imitating Japanese method Chailing decoction granules were superior to Chinese ordinary method granules in curative effects for CRF rats.