日本血吸虫多次感染宿主对治疗的反应性研究

STUDY ON THE RESPONSIBILITY OF PRAZIQUANTEL TO HOST DUE TO RECURRENT INFECTION OF SCHISTOSOMA JAPONICUM

[目的]建立等量血吸虫尾蚴单次感染和多次感染的动物模型,在感染的急性期、慢性期和晚期用吡喹酮治疗,比较这两种感染方式对治疗的反应性。[方法]测量单个虫卵肉芽肿的体积;放免法检测血清中透明质酸(HA);RT-PCR-ELISA半定量测定肝组织中的TGF-β1mRNA,比较两种感染方式治疗组和非治疗组以及治疗组间的差异。[结果]单次感染在急性期经吡喹酮治疗,肉芽肿及纤维化程度显著降低;而多次感染组经吡喹酮治疗后,肉芽肿及纤维化程度无明显改善。[结论]等量的尾蚴,单次感染的治疗效果明显优于多次感染;急性期的治疗效果显著优于慢性期和晚期。

[Objective] To establish animal models of single-infection and multiple-infection of the same number of Schistosoma japonicum.Praziquantel was used to treat this disease at the time of acute stage,chronic phase and advanced stage of infection,and compare the responsibility of the two infectious types.Methods The granulomatous volume of single schistosoma japonicum egg was measured;Hyaluronic acid（HA）in sera and TGF-β1mRNA in livers were detected respectively by radioimmunoassay and RT-PCR-ELISA semi-determinalion,followed by comparing the differences between groups with treatment and without treatment as well as the differences of two groups with treatment.Results The granuloma and degree of fibrosis were significantly decreased after treating with praziquantel in the acute stage when single infection,while not significantly decreased after treating with praziquantel were found when multiple-infection.Conclusion Infected with the same number of cercaria,the efficacy of praziquantel for single-infected is significantly greater than that for multiple-infection;The efficacy in acute stage of infection is significantly better than that in chronic phase and advanced stage.