肥胖男性β_3肾上腺素能受体基因多态性研究

Study on the polymorphisms of β_3 adrenergic receptor gene in obesity males

目的研究单纯性肥胖男性β3肾上腺素能受体(β3-adrenergic receptor,β3-AR)Trp64Arg多态性的分布情况及对肥胖类型的影响。方法利用聚合酶链式反应-限制性片断长度多态性(PCR-RFLP)方法检测单纯性肥胖患者及正常对照β3-AR基因Trp64Arg突变。进一步比较不同类型肥胖中突变的发生率。结果β3-AR基因Trp64Arg突变频率在正常对照组、周围性肥胖组及中心性肥胖患者中分别为16.67%、21.05%、34.78%,Arg等位基因在3组中的频率依次为8.33%、10.53%、19.57%。中心性肥胖患者Trp64Arg突变及Arg等位基因频率较正常对照及周围性肥胖者明显增加,但无统计学差异。结论β3-AR基因Trp64Arg突变可能与男性单纯肥胖者腹内脂肪积聚有关,对此需进一步扩大样本后加以研究。

Objective To explore the polymorphisms of β3-adrenergic receptor （β3-AR） gene Trp64Arg mutation in the simple obesity male subjects of Shenzhen city. Methods 60 male subjects were divided into three groups including norreal control group, peripheral obesity group and central obesity group according to the body mass index and waist-hip ratio （WHR）. The PCR - RFLP method was used to genotype the Trp64Arg mutation in the β3-AR gene. Results Central o- besity group had much higher allelie frequency （34.78%） of the Trp64Arg mutation than peripheral obesity （21.05%） and normal control groups （ 16.67% ） , but no significant differences were found in central obesity subjects compared with periph-eral obesity and control subjects. Conclusion The frequency of Trp64Arg mutation showed an upward tendency in central obesity subjects compared to the other two groups, which indicated that β3-AR gene Trp64Arg mutation may relate to the body fat distribution in the simple obesity male subjects of Shenzhen city.