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彗星试验和微核试验测试广州市颗粒物有机组份遗传毒性 被引量：1

A comparison of comet assay and cell-blocked micronucleus for studying the genotoxicity of organic components in air particulate matters from urban area of Guangzhou
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摘要 目的比较彗星试验和微核试验测试颗粒物提取物遗传毒性的敏感性。方法采集广州市总悬浮颗粒物(TSP)和可吸入颗粒物(PM10)样品,用彗星试验(Comet assay)和微核试验方法(CBMN,Cell Blocked Micronucleus)评价广州市大气颗粒物中不同组份的有机物对淋巴细胞DNA的损伤。结果在0、4、12、20m3eq/ml剂量下彗星试验均呈现剂量-效应关系,而同剂量下微核试验则没有剂量-效应关系。结论在颗粒物提取物的遗传毒性研究中,彗星试验较微核试验敏感。 Objective To compare the sensitivity of comet assay and cell - blocked micronucleus for studying the genotox-icity of extractable matter in air particulate matters. Methods Particulate matters （TSP, PM10）were sampled in urban Guangzhou and the DNA damage potential of extractable organics were study by both comet assay and cell- blocked micronu-cleus （CBMN） on human peripheral blood lymphocyte. Results At doses 0, 4, 12 and 20 m^3 eq/ml significant dose - re-sponse effects were observed for comet assay while no dose - response effects were observed for the CBMN tests at same doses. Conclusion The results indicated that comet assay was much more sensitive than CBMN.
作者 徐海娟 王新明
机构地区 广东省职业病防治院 中国科学院广州地球化学研究所
出处 《中国热带医学》 CAS 2008年第8期1321-1323,共3页 China Tropical Medicine
基金 广东省自然科学基金(06025473) 广州市科技计划(2006Z3-E0721)
关键词 颗粒物 遗传毒性 彗星试验 可提取有机物 Particulate matter, Genotoxieity, Comet assay, Cell- blocked mieronueleus, Extractable organic matter
分类号 R994.6 [医药卫生—毒理学]
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参考文献12

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二级参考文献3

同被引文献23

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中国热带医学
2008年 第8期

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