云南战区部队首次检出类志贺邻单胞菌的生物学鉴定

Biological study on the identification and drug resistance of Plesiomonas shigelloides isolated by army officer of Yunnan Garrion

目的了解云南战区部队感染性腹泻患者的类志贺邻单胞菌(Ps)感染状况与其毒力及药敏谱。方法用SS琼脂平板分离培养,肠道细菌双糖鉴别管初筛,常规生化和VITEK32全自动微生物鉴定仪进行鉴定,电子显微镜观察形态,小白鼠腹腔注射测定毒力。药敏试验采用K-B法。结果从336例腹泻患者粪便中分离出8株Ps菌,检出率为2.38%。全部对小白鼠毒力测定阳性,14种常用抗生素中有9种敏感、2种中度敏感及3种耐药。结论云南战区部队存在Ps感染,该菌毒力较强;药敏谱的明确为部队和地方今后治疗此菌感染提供重要参考。

Objective To identify Plesiomonas shigelloides isolated from stool samples of soldiers with infective dairrhea in Yunnan Garrison Army and determine its virulence as well as drug resistance, Methods Plesiomonas shigelloides was isolated and cultured with SS agar plate and identified with conventional biochemical and VITEK32 automatical apparatus. Its form was observed with electron microscope. Plesiomonas shigelloides was intraperitoneally injected into mice and its virulence was determined with K-B method. Results There 8 Plesiomonas shigelloides strains were islated from 336 samples with a positive rate of 2.38%. The virulence of the islated strains was positive in mice. The sensitive, intermediate sensitive and resistant strains to antibiotics were detemined. Conclusion There is Plesiomonas shigelloides infection in soldiers of Yunnan Garrison Army and the virulence of Plesiomonas shigelloides is relatively strong and the determination of status of drug resistance of this bacterium will offer reference for control of Plesiomonas shigelloides infection in the army and the locality.